caa a22 4500 605480141 CHVBK 20210128100413.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10544-015-0003-5 doi (NATIONALLICENCE)springer-10.1007/s10544-015-0003-5 Acoustophoretic removal of proteins from blood components [Elektronische Daten] [Maria Tenje, Maria Lundgren, Ann-Margret Swärd-Nilsson, Jens Kjeldsen-Kragh, Lena Lyxe, Andreas Lenshof] This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97%, compared to just above 90% of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications. Springer Science+Business Media New York, 2015 Acoustophoresis nationallicence Blood component preparation nationallicence IgA deficiency nationallicence Tenje Maria Department of Biomedical Engineering, Lund University, Lund, Sweden aut Lundgren Maria Department of Clinical Immunology and Transfusion Medicine, Skåne University Hospital, Lund, Sweden aut Swärd-Nilsson Ann-Margret Department of Clinical Immunology and Transfusion Medicine, Skåne University Hospital, Lund, Sweden aut Kjeldsen-Kragh Jens Department of Clinical Immunology and Transfusion Medicine, Skåne University Hospital, Lund, Sweden aut Lyxe Lena Sahlgrenska University Hospital, Göteborg, Sweden aut Lenshof Andreas Department of Biomedical Engineering, Lund University, Lund, Sweden aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-6 1387-2176 17:5<1 2015 17 10544 https://doi.org/10.1007/s10544-015-0003-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-0003-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tenje Maria Department of Biomedical Engineering, Lund University, Lund, Sweden aut NATIONALLICENCE 700 1- Lundgren Maria Department of Clinical Immunology and Transfusion Medicine, Skåne University Hospital, Lund, Sweden aut NATIONALLICENCE 700 1- Swärd-Nilsson Ann-Margret Department of Clinical Immunology and Transfusion Medicine, Skåne University Hospital, Lund, Sweden aut NATIONALLICENCE 700 1- Kjeldsen-Kragh Jens Department of Clinical Immunology and Transfusion Medicine, Skåne University Hospital, Lund, Sweden aut NATIONALLICENCE 700 1- Lyxe Lena Sahlgrenska University Hospital, Göteborg, Sweden aut NATIONALLICENCE 700 1- Lenshof Andreas Department of Biomedical Engineering, Lund University, Lund, Sweden aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-6 1387-2176 17:5<1 2015 17 10544