caa a22 4500 605480176 CHVBK 20210128100413.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10544-015-9991-4 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9991-4 The development of non-toxic ionic-crosslinked chitosan-based microspheres as carriers for the controlled release of silk sericin [Elektronische Daten] [Pornanong Aramwit, Sanong Ekasit, Rungnapha Yamdech] Silk sericin is recently shown to possess various biological activities for biomedical applications. While various sericin carriers were developed for drug delivery system, very few researches considered sericin as a bioactive molecule itself. In this study, sericin incorporated in the chitosan-based microspheres was introduced as a bioactive molecule and bioactive carrier at the same time. The chitosan/sericin (CH/SS) microspheres at different composition (80/20, 70/30, 60/40, and 50/50) were successfully fabricated using anhydroustri-polyphosphate (TPP) as a polyanionic crosslinker. The microspheres with an average size of 1-4μm and narrow size distribution were obtained. From FT-IR spectra, the presence of both chitosan and sericin in the microspheres confirmed the occurrence of ionic interaction that crosslink them within the microspheres. We also found that the CH/SS microspheres prepared at 50/50 could encapsulate sericin at the highest percentage (37.28%) and release sericin in the most sustained behavior, possibly due to the strong ionic interaction of the positively charged chitosan and the negatively charged sericin. On the other hand, the composition of CH/SS had no effect on the degradation rate of microspheres. All microspheres continuously degraded and remained around 20% after 14days of enzymatic degradation. This explained that the ionic crosslinkings between chitosan and sericin could be demolished by the enzyme and hydrolysis. Furthermore, we have verified that all CH/SS microspheres at any concentrations showed non-toxicity to L929 mouse fibroblast cells. Therefore, we suggested that the non-toxic ionic-crosslinked CH/SS microspheres could be incorporated in wound dressing material to achieve the sustained release of sericin for accelerated wound healing. Springer Science+Business Media New York, 2015 Sericin nationallicence Chitosan nationallicence Tri-polyphosphate nationallicence Microspheres nationallicence Ionic crosslinking nationallicence Controlled release nationallicence Aramwit Pornanong Bioactive Resources for Innovative Clinical Applications Research Unit and Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhayaThai Road, 10330, Bangkok, Phatumwan, Thailand aut Ekasit Sanong Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 PhyaThai Road, 10330, Bangkok, Patumwan, Thailand aut Yamdech Rungnapha Bioactive Resources for Innovative Clinical Applications Research Unit and Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhayaThai Road, 10330, Bangkok, Phatumwan, Thailand aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-9 1387-2176 17:5<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9991-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9991-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aramwit Pornanong Bioactive Resources for Innovative Clinical Applications Research Unit and Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhayaThai Road, 10330, Bangkok, Phatumwan, Thailand aut NATIONALLICENCE 700 1- Ekasit Sanong Sensor Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 PhyaThai Road, 10330, Bangkok, Patumwan, Thailand aut NATIONALLICENCE 700 1- Yamdech Rungnapha Bioactive Resources for Innovative Clinical Applications Research Unit and Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhayaThai Road, 10330, Bangkok, Phatumwan, Thailand aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-9 1387-2176 17:5<1 2015 17 10544