Dielectrophoretic characterization of cells in a stationary nanoliter droplet array with generated chemical gradients
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| 001 | 605480184 | ||
| 003 | CHVBK | ||
| 005 | 20210128100413.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20151001xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s10544-015-9996-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9996-z | ||
| 245 | 0 | 0 | |a Dielectrophoretic characterization of cells in a stationary nanoliter droplet array with generated chemical gradients |h [Elektronische Daten] |c [Tom Ben-Arye, Sinwook Park, Jonathan Shemesh, Dan Peer, Shulamit Levenberg, Gilad Yossifon] |
| 520 | 3 | |a A novel design of reusable microfluidic platform that generates a stationary nanoliter droplet array (SNDA) for cell incubation and analysis, equipped with a complementary array of individually addressable electrodes for each microwell is studied. Various solute concentration gradients were generated between the wells where dielectrophoresis (DEP) was used to characterize the effect of the gradients on the cell's response. The feasibility of generating concentration gradients and observation of DEP responses was demonstrated using a gradient of salts in combination with microparticles and viable cells. L1210 Lymphoma cells were used as the model cells in these experiments. Lymphoma cells' cross-over frequency (COF) decreased with increasing stress conditions. Specifically, a linear decrease in the cell COF was measured as a function of solution tonicity and blebbistatin dose. Lymphoma cells were incubated under a gradient of the chemotherapeutic agent doxorubicin (DOX), which led to saturation in the cell-COF response at 30nM DOX, demonstrating the potential of the platform in screening of label-free drugs. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Microfluidics |2 nationallicence | |
| 690 | 7 | |a Cell analysis |2 nationallicence | |
| 690 | 7 | |a Dielectrophoresis |2 nationallicence | |
| 700 | 1 | |a Ben-Arye |D Tom |u Department of Biomedical Engineering, Technion, 32000, Haifa, Israel |4 aut | |
| 700 | 1 | |a Park |D Sinwook |u Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion, 32000, Haifa, Israel |4 aut | |
| 700 | 1 | |a Shemesh |D Jonathan |u Department of Mechanical and Manufacturing Engineering, UNSW Australia, Sydney, NSW, Australia |4 aut | |
| 700 | 1 | |a Peer |D Dan |u Laboratory of NanoMedicine, Department of Cell Research and Immunology, Department of Material Science and Engineering, and the Center for Nanoscience and Nanotechnology, Tel Aviv University, 69978, Tel Aviv, Israel |4 aut | |
| 700 | 1 | |a Levenberg |D Shulamit |u Department of Biomedical Engineering, Technion, 32000, Haifa, Israel |4 aut | |
| 700 | 1 | |a Yossifon |D Gilad |u Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion, 32000, Haifa, Israel |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/5(2015-10-01), 1-10 |x 1387-2176 |q 17:5<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9996-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9996-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ben-Arye |D Tom |u Department of Biomedical Engineering, Technion, 32000, Haifa, Israel |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Park |D Sinwook |u Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion, 32000, Haifa, Israel |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shemesh |D Jonathan |u Department of Mechanical and Manufacturing Engineering, UNSW Australia, Sydney, NSW, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Peer |D Dan |u Laboratory of NanoMedicine, Department of Cell Research and Immunology, Department of Material Science and Engineering, and the Center for Nanoscience and Nanotechnology, Tel Aviv University, 69978, Tel Aviv, Israel |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Levenberg |D Shulamit |u Department of Biomedical Engineering, Technion, 32000, Haifa, Israel |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yossifon |D Gilad |u Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion, 32000, Haifa, Israel |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/5(2015-10-01), 1-10 |x 1387-2176 |q 17:5<1 |1 2015 |2 17 |o 10544 | ||