caa a22 4500 605480184 CHVBK 20210128100413.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10544-015-9996-z doi (NATIONALLICENCE)springer-10.1007/s10544-015-9996-z Dielectrophoretic characterization of cells in a stationary nanoliter droplet array with generated chemical gradients [Elektronische Daten] [Tom Ben-Arye, Sinwook Park, Jonathan Shemesh, Dan Peer, Shulamit Levenberg, Gilad Yossifon] A novel design of reusable microfluidic platform that generates a stationary nanoliter droplet array (SNDA) for cell incubation and analysis, equipped with a complementary array of individually addressable electrodes for each microwell is studied. Various solute concentration gradients were generated between the wells where dielectrophoresis (DEP) was used to characterize the effect of the gradients on the cell's response. The feasibility of generating concentration gradients and observation of DEP responses was demonstrated using a gradient of salts in combination with microparticles and viable cells. L1210 Lymphoma cells were used as the model cells in these experiments. Lymphoma cells' cross-over frequency (COF) decreased with increasing stress conditions. Specifically, a linear decrease in the cell COF was measured as a function of solution tonicity and blebbistatin dose. Lymphoma cells were incubated under a gradient of the chemotherapeutic agent doxorubicin (DOX), which led to saturation in the cell-COF response at 30nM DOX, demonstrating the potential of the platform in screening of label-free drugs. Springer Science+Business Media New York, 2015 Microfluidics nationallicence Cell analysis nationallicence Dielectrophoresis nationallicence Ben-Arye Tom Department of Biomedical Engineering, Technion, 32000, Haifa, Israel aut Park Sinwook Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion, 32000, Haifa, Israel aut Shemesh Jonathan Department of Mechanical and Manufacturing Engineering, UNSW Australia, Sydney, NSW, Australia aut Peer Dan Laboratory of NanoMedicine, Department of Cell Research and Immunology, Department of Material Science and Engineering, and the Center for Nanoscience and Nanotechnology, Tel Aviv University, 69978, Tel Aviv, Israel aut Levenberg Shulamit Department of Biomedical Engineering, Technion, 32000, Haifa, Israel aut Yossifon Gilad Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion, 32000, Haifa, Israel aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-10 1387-2176 17:5<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9996-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9996-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ben-Arye Tom Department of Biomedical Engineering, Technion, 32000, Haifa, Israel aut NATIONALLICENCE 700 1- Park Sinwook Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion, 32000, Haifa, Israel aut NATIONALLICENCE 700 1- Shemesh Jonathan Department of Mechanical and Manufacturing Engineering, UNSW Australia, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Peer Dan Laboratory of NanoMedicine, Department of Cell Research and Immunology, Department of Material Science and Engineering, and the Center for Nanoscience and Nanotechnology, Tel Aviv University, 69978, Tel Aviv, Israel aut NATIONALLICENCE 700 1- Levenberg Shulamit Department of Biomedical Engineering, Technion, 32000, Haifa, Israel aut NATIONALLICENCE 700 1- Yossifon Gilad Faculty of Mechanical Engineering, Micro- and Nanofluidics Laboratory, Technion, 32000, Haifa, Israel aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/5(2015-10-01), 1-10 1387-2176 17:5<1 2015 17 10544