Microfluidic-aided genotyping of zebrafish in the first 48h with 100% viability
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| 005 | 20210128100414.0 | ||
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| 024 | 7 | 0 | |a 10.1007/s10544-015-9946-9 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9946-9 | ||
| 245 | 0 | 0 | |a Microfluidic-aided genotyping of zebrafish in the first 48h with 100% viability |h [Elektronische Daten] |c [Raheel Samuel, Regan Stephenson, Paula Roy, Rob Pryor, Luming Zhou, Joshua Bonkowsky, Bruce Gale] |
| 520 | 3 | |a This paper introduces an innovative method for genotyping 1-2days old zebrafish embryos, without sacrificing the life/health of the embryos. The method utilizes microfluidic technology to extract and collect a small amount of genetic material from the chorionic fluid or fin tissue of the embryo. Then, using conventional DNA extraction, PCR amplification, and high resolution melt analysis with fluorescent DNA detection techniques, the embryo is genotyped. The chorionic fluid approach was successful 78% of the time while the fin clipping method was successful 100% of the time. Chorionic fluid was shown to only contain DNA from the embryo and not from the mother. These results suggest a novel method to genotype zebrafish embryos that can facilitate high-throughput screening, while maintaining 100% viability of the embryo. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Zebrafish |2 nationallicence | |
| 690 | 7 | |a Genotyping |2 nationallicence | |
| 690 | 7 | |a Microfluidics |2 nationallicence | |
| 700 | 1 | |a Samuel |D Raheel |u Department of Mechanical Engineering, University of Utah, 84112, Salt Lake City, UT, USA |4 aut | |
| 700 | 1 | |a Stephenson |D Regan |u Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | |
| 700 | 1 | |a Roy |D Paula |u Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | |
| 700 | 1 | |a Pryor |D Rob |u Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | |
| 700 | 1 | |a Zhou |D Luming |u Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | |
| 700 | 1 | |a Bonkowsky |D Joshua |u Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | |
| 700 | 1 | |a Gale |D Bruce |u Department of Mechanical Engineering, University of Utah, 84112, Salt Lake City, UT, USA |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-7 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9946-9 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9946-9 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Samuel |D Raheel |u Department of Mechanical Engineering, University of Utah, 84112, Salt Lake City, UT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Stephenson |D Regan |u Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Roy |D Paula |u Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pryor |D Rob |u Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Luming |u Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bonkowsky |D Joshua |u Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gale |D Bruce |u Department of Mechanical Engineering, University of Utah, 84112, Salt Lake City, UT, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-7 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | ||