caa a22 4500 605480214 CHVBK 20210128100414.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10544-015-9946-9 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9946-9 Microfluidic-aided genotyping of zebrafish in the first 48h with 100% viability [Elektronische Daten] [Raheel Samuel, Regan Stephenson, Paula Roy, Rob Pryor, Luming Zhou, Joshua Bonkowsky, Bruce Gale] This paper introduces an innovative method for genotyping 1-2days old zebrafish embryos, without sacrificing the life/health of the embryos. The method utilizes microfluidic technology to extract and collect a small amount of genetic material from the chorionic fluid or fin tissue of the embryo. Then, using conventional DNA extraction, PCR amplification, and high resolution melt analysis with fluorescent DNA detection techniques, the embryo is genotyped. The chorionic fluid approach was successful 78% of the time while the fin clipping method was successful 100% of the time. Chorionic fluid was shown to only contain DNA from the embryo and not from the mother. These results suggest a novel method to genotype zebrafish embryos that can facilitate high-throughput screening, while maintaining 100% viability of the embryo. Springer Science+Business Media New York, 2015 Zebrafish nationallicence Genotyping nationallicence Microfluidics nationallicence Samuel Raheel Department of Mechanical Engineering, University of Utah, 84112, Salt Lake City, UT, USA aut Stephenson Regan Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA aut Roy Paula Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA aut Pryor Rob Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA aut Zhou Luming Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA aut Bonkowsky Joshua Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA aut Gale Bruce Department of Mechanical Engineering, University of Utah, 84112, Salt Lake City, UT, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-7 1387-2176 17:2<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9946-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9946-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Samuel Raheel Department of Mechanical Engineering, University of Utah, 84112, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- Stephenson Regan Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- Roy Paula Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- Pryor Rob Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- Zhou Luming Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- Bonkowsky Joshua Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- Gale Bruce Department of Mechanical Engineering, University of Utah, 84112, Salt Lake City, UT, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-7 1387-2176 17:2<1 2015 17 10544