Highly efficient capture and harvest of circulating tumor cells on a microfluidic chip integrated with herringbone and micropost arrays
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605480222 | ||
| 003 | CHVBK | ||
| 005 | 20210128100414.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150401xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s10544-015-9945-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9945-x | ||
| 245 | 0 | 0 | |a Highly efficient capture and harvest of circulating tumor cells on a microfluidic chip integrated with herringbone and micropost arrays |h [Elektronische Daten] |c [Peng Xue, Yafeng Wu, Jinhong Guo, Yuejun Kang] |
| 520 | 3 | |a Circulating tumor cells (CTCs), which are derived from primary tumor site and transported to distant organs, are considered as the major cause of metastasis. So far, various techniques have been applied for CTC isolation and enumeration. However, there exists great demand to improve the sensitivity of CTC capture, and it remains challenging to elute the cells efficiently from device for further biomolecular and cellular analyses. In this study, we fabricate a dual functional chip integrated with herringbone structure and micropost array to achieve CTC capture and elution through EpCAM-based immunoreaction. Hep3B tumor cell line is selected as the model of CTCs for processing using this device. The results demonstrate that the capture limit of Hep3B cells can reach up to 10 cells (per mL of sample volume) with capture efficiency of 80% on average. Moreover, the elution rate of the captured Hep3B cells can reach up to 69.4% on average for cell number ranging from 1 to 100. These results demonstrate that this device exhibits dual functions with considerably high capture rate and elution rate, indicating its promising capability for cancer diagnosis and therapeutics. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Circulating tumor cells |2 nationallicence | |
| 690 | 7 | |a Herringbone |2 nationallicence | |
| 690 | 7 | |a Micropost |2 nationallicence | |
| 690 | 7 | |a Cell capture and harvest |2 nationallicence | |
| 700 | 1 | |a Xue |D Peng |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | |
| 700 | 1 | |a Wu |D Yafeng |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | |
| 700 | 1 | |a Guo |D Jinhong |u Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 610054, Chengdu, People's Republic of China |4 aut | |
| 700 | 1 | |a Kang |D Yuejun |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-8 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9945-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9945-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xue |D Peng |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Yafeng |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Guo |D Jinhong |u Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 610054, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kang |D Yuejun |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-8 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | ||