caa a22 4500 605480222 CHVBK 20210128100414.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10544-015-9945-x doi (NATIONALLICENCE)springer-10.1007/s10544-015-9945-x Highly efficient capture and harvest of circulating tumor cells on a microfluidic chip integrated with herringbone and micropost arrays [Elektronische Daten] [Peng Xue, Yafeng Wu, Jinhong Guo, Yuejun Kang] Circulating tumor cells (CTCs), which are derived from primary tumor site and transported to distant organs, are considered as the major cause of metastasis. So far, various techniques have been applied for CTC isolation and enumeration. However, there exists great demand to improve the sensitivity of CTC capture, and it remains challenging to elute the cells efficiently from device for further biomolecular and cellular analyses. In this study, we fabricate a dual functional chip integrated with herringbone structure and micropost array to achieve CTC capture and elution through EpCAM-based immunoreaction. Hep3B tumor cell line is selected as the model of CTCs for processing using this device. The results demonstrate that the capture limit of Hep3B cells can reach up to 10 cells (per mL of sample volume) with capture efficiency of 80% on average. Moreover, the elution rate of the captured Hep3B cells can reach up to 69.4% on average for cell number ranging from 1 to 100. These results demonstrate that this device exhibits dual functions with considerably high capture rate and elution rate, indicating its promising capability for cancer diagnosis and therapeutics. Springer Science+Business Media New York, 2015 Circulating tumor cells nationallicence Herringbone nationallicence Micropost nationallicence Cell capture and harvest nationallicence Xue Peng School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut Wu Yafeng School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut Guo Jinhong Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 610054, Chengdu, People's Republic of China aut Kang Yuejun School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-8 1387-2176 17:2<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9945-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9945-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xue Peng School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut NATIONALLICENCE 700 1- Wu Yafeng School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut NATIONALLICENCE 700 1- Guo Jinhong Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, 610054, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Kang Yuejun School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-8 1387-2176 17:2<1 2015 17 10544