Tapered-slit membrane filters for high-throughput viable circulating tumor cell isolation
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| 008 | 210128e20150401xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s10544-015-9949-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-015-9949-6 | ||
| 245 | 0 | 0 | |a Tapered-slit membrane filters for high-throughput viable circulating tumor cell isolation |h [Elektronische Daten] |c [Yoon-Tae Kang, Il Doh, Young-Ho Cho] |
| 520 | 3 | |a This paper presents tapered-slit membrane filters for high-throughput viable circulating tumor cell (CTC) isolation. The membrane filter with a 2D array of vertical tapered slits with a gap that is wide at the entrance and gradually decreases with depth, provide minimal cell stress and reduce 82.14% of the stress generated in conventional straight-hole filters. We designed two types of tapered-slit filters, Filters 6 and 8, respectively, containing the tapered slits with outlet widths of 6μm and 8μm at a slit density of 34,445/cm2 on the membrane. We fabricated the vertical slits with a tapered angle of 2 ° on a SU8 membrane by adjusting the UV expose dose and the air gap between the membrane and the photomask during lithography. In the experimental study, the proposed tapered-slit filter captured 89.87% and 82.44% of the cancer cells spiked in phosphate buffered saline (PBS) and diluted blood (blood: PBS = 1:4), respectively, at a sample flow rate of 5ml per hour, which is 33.3 times faster than previous lateral tapered-slit filters. We further verified the capability to culture on chip after capturing: 72.33% of cells among the captured cells still remained viable after a 5-day culture. The proposed tapered-slit membrane filters verified high-throughput viable CTC isolation capability, thereby inaugurating further advanced CTC research for cancer diagnosis and prognosis. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Circulating tumor cells |2 nationallicence | |
| 690 | 7 | |a Viable isolation |2 nationallicence | |
| 690 | 7 | |a Tapered-slit filter |2 nationallicence | |
| 700 | 1 | |a Kang |D Yoon-Tae |u Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea |4 aut | |
| 700 | 1 | |a Doh |D Il |u Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea |4 aut | |
| 700 | 1 | |a Cho |D Young-Ho |u Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-7 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9949-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9949-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kang |D Yoon-Tae |u Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Doh |D Il |u Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cho |D Young-Ho |u Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-7 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | ||