caa a22 4500 605480257 CHVBK 20210128100414.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10544-015-9944-y doi (NATIONALLICENCE)springer-10.1007/s10544-015-9944-y Drug coated microneedles for minimally-invasive treatment of oral carcinomas: development and in vitro evaluation [Elektronische Daten] [Yunzhe Ma, Seth Boese, Zhen Luo, Nitin Nitin, Harvinder Gill] Treatment of recurring oral cancers is challenging as common surgical approaches are not feasible for these patients. In addition, these patients do not respond well to systemic chemotherapy. Localized intratumoral injection of anti-cancer drugs is considered to be an attractive alternative treatment approach for these patients. However, conventional hypodermic injections result in poor distribution of the drug in the tumor and leakage of the drug from the injection site to systemic circulation, in addition to causing pain to the patient. The objective of this study was to develop coated microneedles as a novel device for direct and minimally invasive intratumoral delivery of anti-cancer drugs. Poly(lactic-co-glycolic) acid (PLGA) nanoparticles encapsulating doxorubicin (DOX) were prepared and coated on inplane (1D) microneedles. Microscopic evaluation of 3D tissue phantoms and porcine cadaver buccal tissues that were treated with 1D microneedle arrays coated with DOX-PLGA nanoparticles demonstrated that DOX could diffuse both laterally and vertically in to the tissues and produced cellular cytotoxicity. Out of plane (2D) microneedle arrays measuring 1cm x 1cm with 57 microneedles coated with free DOX could produce uniform distribution of DOX in a porcine cadaver buccal tissue up to a depth greater than 3mm. Hypodermic injection of different volumes in to a porcine buccal tissue confirmed significant leakage of the injected volume (about 25% of the injected 80μl). In summary, this study demonstrates that drug coated microneedles is an attractive microscale device that can uniformly and effectively deliver drugs to localized oral cancers. This microscale device has potential to impact the treatment of oral cancer patients. Springer Science+Business Media New York, 2015 Coated microneedles nationallicence Doxorubicin nationallicence Intratumoral nationallicence Nanoparticles nationallicence PLGA nationallicence Ma Yunzhe Department of Chemical Engineering, Texas Tech University, 8th Street & Canton Ave, 79409-3121, Lubbock, TX, USA aut Boese Seth Department of Chemical Engineering, Texas Tech University, 8th Street & Canton Ave, 79409-3121, Lubbock, TX, USA aut Luo Zhen Department of Biological and Agricultural Engineering, University of California, 95616, Davis, CA, USA aut Nitin Nitin Department of Biological and Agricultural Engineering, University of California, 95616, Davis, CA, USA aut Gill Harvinder Department of Chemical Engineering, Texas Tech University, 8th Street & Canton Ave, 79409-3121, Lubbock, TX, USA aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-14 1387-2176 17:2<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9944-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9944-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Yunzhe Department of Chemical Engineering, Texas Tech University, 8th Street & Canton Ave, 79409-3121, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Boese Seth Department of Chemical Engineering, Texas Tech University, 8th Street & Canton Ave, 79409-3121, Lubbock, TX, USA aut NATIONALLICENCE 700 1- Luo Zhen Department of Biological and Agricultural Engineering, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Nitin Nitin Department of Biological and Agricultural Engineering, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Gill Harvinder Department of Chemical Engineering, Texas Tech University, 8th Street & Canton Ave, 79409-3121, Lubbock, TX, USA aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-14 1387-2176 17:2<1 2015 17 10544