Quantification of nanoparticle endocytosis based on double fluorescent pH-sensitive nanoparticles
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| 024 | 7 | 0 | |a 10.1007/s10544-015-9947-8 |2 doi |
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| 245 | 0 | 0 | |a Quantification of nanoparticle endocytosis based on double fluorescent pH-sensitive nanoparticles |h [Elektronische Daten] |c [Andréa Kurtz-Chalot, Jean-Philippe Klein, Jérémie Pourchez, Delphine Boudard, Valérie Bin, Odile Sabido, Laurence Marmuse, Michèle Cottier, Valérie Forest] |
| 520 | 3 | |a Amorphous silica is a particularly interesting material because of its inertness and chemical stability. Silica nanoparticles have been recently developed for biomedical purposes but their innocuousness must be carefully investigated before clinical use. The relationship between nanoparticles physicochemical features, their uptake by cells and their biological activity represents a crucial issue, especially for the development of nanomedicine. This work aimed at adapting a method for the quantification of nanoparticle endocytosis based on pH-sensitive and double fluorescent particles. For that purpose, silica nanoparticles containing two fluorophores: FITC and pHrodoTM were developed, their respective fluorescence emission depends on the external pH. Indeed, FITC emits a green fluorescence at physiological pH and pHrodoTM emits a red fluorescence which intensity increased with acidification. Therefore, nanoparticles remained outside the cells could be clearly distinguished from nanoparticles uptaken by cells as these latter could be spotted inside cellular acidic compartments (such as phagolysosomes, micropinosomes ). Using this model, the endocytosis of 60nm nanoparticles incubated with the RAW 264.7 macrophages was quantified using time-lapse microscopy and compared to that of 130nm submicronic particles. The amount of internalized particles was also evaluated by fluorimetry. The biological impact of the particles was also investigated in terms of cytotoxicity, pro-inflammatory response and oxidative stress. Results clearly demonstrated that nanoparticles were more uptaken and more reactive than submicronic particles. Moreover, we validated a method of endocytosis quantification. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Silica particles |2 nationallicence | |
| 690 | 7 | |a Macrophages |2 nationallicence | |
| 690 | 7 | |a Size |2 nationallicence | |
| 690 | 7 | |a Cellular uptake |2 nationallicence | |
| 690 | 7 | |a Cytotoxicity |2 nationallicence | |
| 700 | 1 | |a Kurtz-Chalot |D Andréa |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | |
| 700 | 1 | |a Klein |D Jean-Philippe |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | |
| 700 | 1 | |a Pourchez |D Jérémie |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | |
| 700 | 1 | |a Boudard |D Delphine |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | |
| 700 | 1 | |a Bin |D Valérie |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | |
| 700 | 1 | |a Sabido |D Odile |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | |
| 700 | 1 | |a Marmuse |D Laurence |u Nano-H, 2 Place de l'Europe, 38070, Saint Quentin Fallavier, France |4 aut | |
| 700 | 1 | |a Cottier |D Michèle |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | |
| 700 | 1 | |a Forest |D Valérie |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-12 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-015-9947-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-015-9947-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kurtz-Chalot |D Andréa |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Klein |D Jean-Philippe |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Pourchez |D Jérémie |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Boudard |D Delphine |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bin |D Valérie |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sabido |D Odile |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Marmuse |D Laurence |u Nano-H, 2 Place de l'Europe, 38070, Saint Quentin Fallavier, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cottier |D Michèle |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Forest |D Valérie |u LINA Laboratoire Interdisciplinaire d'étude des Nanoparticules Aérosolisées (EA 4624), 15 rue Ambroise Paré, 42023, Saint-Étienne, Cedex 2, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-12 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | ||