caa a22 4500 605480338 CHVBK 20210128100415.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10544-015-9939-8 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9939-8 A microfluidic device for efficient chemical testing using Caenorhabditis elegans [Elektronische Daten] [Pengfei Song, Weize Zhang, Alexandre Sobolevski, Kristine Bernard, Siegfried Hekimi, Xinyu Liu] The nematode worm Caenorhabditis elegans has been employed as a popular model organism in many fields of biological research. In this paper, we present a microfluidic device for facilitating chemical testing using C. elegans. For testing chemicals on chip, the device houses single nematodes in microfluidic chambers and precisely adjusts the chamber's chemical environment during experiments. Eight nematodes can be readily loaded into the chambers through separate loading channels in a quick and gentle manner. In addition, a custom-made software with a graphic user interface is also created for quantitative analysis of locomotion parameters (swimming frequency and bend amplitude) of the nematodes in response to chemical stimuli, thus greatly enhancing the efficiency of data collection. We perform proof-of-concept experiments using two chemicals, zinc ion (Zn2+) and glucose, to demonstrate the effectiveness of the microfluidic device. Springer Science+Business Media New York, 2015 Microfluidics nationallicence Chemical testing nationallicence C. elegans nationallicence Image processing nationallicence Behavior studies nationallicence Locomotion nationallicence Song Pengfei Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, H3A 0C3, Montreal, Quebec, Canada aut Zhang Weize Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, H3A 0C3, Montreal, Quebec, Canada aut Sobolevski Alexandre Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, H3A 0C3, Montreal, Quebec, Canada aut Bernard Kristine Department of Biology, McGill University, 1205 Docteur Penfield, H3A 1B1, Montreal, Quebec, Canada aut Hekimi Siegfried Department of Biology, McGill University, 1205 Docteur Penfield, H3A 1B1, Montreal, Quebec, Canada aut Liu Xinyu Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, H3A 0C3, Montreal, Quebec, Canada aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-10 1387-2176 17:2<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9939-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9939-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Pengfei Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, H3A 0C3, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Zhang Weize Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, H3A 0C3, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Sobolevski Alexandre Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, H3A 0C3, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Bernard Kristine Department of Biology, McGill University, 1205 Docteur Penfield, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Hekimi Siegfried Department of Biology, McGill University, 1205 Docteur Penfield, H3A 1B1, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Liu Xinyu Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, H3A 0C3, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-10 1387-2176 17:2<1 2015 17 10544