caa a22 4500 605480346 CHVBK 20210128100415.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10544-015-9928-y doi (NATIONALLICENCE)springer-10.1007/s10544-015-9928-y 3D printing of soft lithography mold for rapid production of polydimethylsiloxane-based microfluidic devices for cell stimulation with concentration gradients [Elektronische Daten] [Ken-ichiro Kamei, Yasumasa Mashimo, Yoshie Koyama, Christopher Fockenberg, Miyuki Nakashima, Minako Nakajima, Junjun Li, Yong Chen] Three-dimensional (3D) printing is advantageous over conventional technologies for the fabrication of sophisticated structures such as 3D micro-channels for future applications in tissue engineering and drug screening. We aimed to apply this technology to cell-based assays using polydimethylsiloxane (PDMS), the most commonly used material for fabrication of micro-channels used for cell culture experiments. Useful properties of PDMS include biocompatibility, gas permeability and transparency. We developed a simple and robust protocol to generate PDMS-based devices using a soft lithography mold produced by 3D printing. 3D chemical gradients were then generated to stimulate cells confined to a micro-channel. We demonstrate that concentration gradients of growth factors, important regulators of cell/tissue functions in vivo, influence the survival and growth of human embryonic stem cells. Thus, this approach for generation of 3D concentration gradients could have strong implications for tissue engineering and drug screening. Springer Science+Business Media New York, 2015 3D printing nationallicence Microfluidics nationallicence Polydimethylsiloxane nationallicence Human embryonic stem cell nationallicence Concentration gradient nationallicence Kamei Ken-ichiro Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut Mashimo Yasumasa Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut Koyama Yoshie Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut Fockenberg Christopher Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut Nakashima Miyuki Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut Nakajima Minako Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut Li Junjun Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut Chen Yong Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-8 1387-2176 17:2<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9928-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9928-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamei Ken-ichiro Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Mashimo Yasumasa Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Koyama Yoshie Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Fockenberg Christopher Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Nakashima Miyuki Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Nakajima Minako Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Li Junjun Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut NATIONALLICENCE 700 1- Chen Yong Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 606-8501, Kyoto, Japan aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-8 1387-2176 17:2<1 2015 17 10544