caa a22 4500 605480354 CHVBK 20210128100415.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10544-015-9933-1 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9933-1 Optical assembly of bio-hybrid micro-robots [Elektronische Daten] [Álvaro Barroso, Shirin Landwerth, Mike Woerdemann, Christina Alpmann, Tim Buscher, Maike Becker, Armido Studer, Cornelia Denz] The combination of micro synthetic structures with bacterial flagella motors represents an actual trend for the construction of self-propelled micro-robots. The development of methods for fabrication of these bacteria-based robots is a first crucial step towards the realization of functional miniature and autonomous moving robots. We present a novel scheme based on optical trapping to fabricate living micro-robots. By using holographic optical tweezers that allow three-dimensional manipulation in real time, we are able to arrange the building blocks that constitute the micro-robot in a defined way. We demonstrate exemplarily that our method enables the controlled assembly of living micro-robots consisting of a rod-shaped prokaryotic bacterium and a single elongated zeolite L crystal, which are used as model of the biological and abiotic components, respectively. We present different proof-of-principle approaches for the site-selective attachment of the bacteria on the particle surface. The propulsion of the optically assembled micro-robot demonstrates the potential of the proposed method as a powerful strategy for the fabrication of bio-hybrid micro-robots. The Author(s), 2015 Bio-hybrid systems nationallicence Micro-robots nationallicence Holographic optical tweezers nationallicence Non-spherical shape nationallicence Zeolite L nanocontainers nationallicence Micromanipulation nationallicence Barroso Álvaro Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut Landwerth Shirin Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut Woerdemann Mike Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut Alpmann Christina Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut Buscher Tim Organic Chemistry Institute, Westfälische Wilhems Universität, Correnstrasse 40, 48149, Muenster, Germany aut Becker Maike Organic Chemistry Institute, Westfälische Wilhems Universität, Correnstrasse 40, 48149, Muenster, Germany aut Studer Armido Organic Chemistry Institute, Westfälische Wilhems Universität, Correnstrasse 40, 48149, Muenster, Germany aut Denz Cornelia Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-8 1387-2176 17:2<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9933-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9933-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barroso Álvaro Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut NATIONALLICENCE 700 1- Landwerth Shirin Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut NATIONALLICENCE 700 1- Woerdemann Mike Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut NATIONALLICENCE 700 1- Alpmann Christina Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut NATIONALLICENCE 700 1- Buscher Tim Organic Chemistry Institute, Westfälische Wilhems Universität, Correnstrasse 40, 48149, Muenster, Germany aut NATIONALLICENCE 700 1- Becker Maike Organic Chemistry Institute, Westfälische Wilhems Universität, Correnstrasse 40, 48149, Muenster, Germany aut NATIONALLICENCE 700 1- Studer Armido Organic Chemistry Institute, Westfälische Wilhems Universität, Correnstrasse 40, 48149, Muenster, Germany aut NATIONALLICENCE 700 1- Denz Cornelia Institute of Applied Physics, Westfälische Wilhems Universität, Correnstrasse 2-4, 48149, Muenster, Germany aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-8 1387-2176 17:2<1 2015 17 10544