caa a22 4500 605480362 CHVBK 20210128100415.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10544-015-9950-0 doi (NATIONALLICENCE)springer-10.1007/s10544-015-9950-0 A soft, stretchable and conductive biointerface for cell mechanobiology [Elektronische Daten] [Irene Bernardeschi, Francesco Greco, Gianni Ciofani, Attilio Marino, Virgilio Mattoli, Barbara Mazzolai, Lucia Beccai] In mechanobiology the study of cell response to mechanical stimuli is fundamental, and the involved processes (i.e., mechanotransduction) need to be investigated by interfacing (mechanically and electrically) with the cells in dynamic and non-invasive natural-like conditions. In this work, we present a novel soft, stretchable and conductive biointerface that allows both cell mechanical stimulation and dynamic impedance recording. The biointerface stretchability and conductivity, jointly to the biocompatibility and transparency needed to perform cell culture studies, were obtained by exploiting the formation of wrinkles on the surface of a 90nm thick conductive layer of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) on a pre-stretched 130μm thick poly(dimethylsiloxane) (PDMS) substrate. Cell adhesion and proliferation of SH-SY5Y human neuroblastoma cells were evaluated, and cell differentiation on the corrugated surface was assessed. We demonstrate how the biointerface remains conductive when applying uniaxial strain up to 10%, and when cell culturing is performed. Finally, a reduction of about 30% of the relative impedance variation signal was measured, with respect to the control, as a result of the mechanical stimulation of cells. Springer Science+Business Media New York, 2015 Stretchable biointerface nationallicence Impedance nationallicence Biohybrid nationallicence Surface wrinkling nationallicence PEDOT:PSS nationallicence Mechanotransduction nationallicence Bernardeschi Irene Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut Greco Francesco Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut Ciofani Gianni Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut Marino Attilio Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut Mattoli Virgilio Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut Mazzolai Barbara Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut Beccai Lucia Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-11 1387-2176 17:2<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9950-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9950-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bernardeschi Irene Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut NATIONALLICENCE 700 1- Greco Francesco Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut NATIONALLICENCE 700 1- Ciofani Gianni Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut NATIONALLICENCE 700 1- Marino Attilio Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut NATIONALLICENCE 700 1- Mattoli Virgilio Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut NATIONALLICENCE 700 1- Mazzolai Barbara Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut NATIONALLICENCE 700 1- Beccai Lucia Center for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, PI, Italy aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-11 1387-2176 17:2<1 2015 17 10544