caa a22 4500 605480370 CHVBK 20210128100415.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10544-015-9929-x doi (NATIONALLICENCE)springer-10.1007/s10544-015-9929-x Towards the fabrication of artificial 3D microdevices for neural cell networks [Elektronische Daten] [Andrew Gill, Ílida Ortega, Stephen Kelly, Frederik Claeyssens] This work reports first steps towards the development of artificial neural stem cell microenvironments for the control and assessment of neural stem cell behaviour. Stem cells have been shown to be found in specific, supportive microenvironments (niches) and are believed to play an important role in tissue regeneration mechanisms. These environments are intricate spaces with chemical and biological features. Here we present work towards the development of physically defined microdevices in which neural and neural stem cells can be studied in 3-dimensions. We have approached this challenge by creating bespoke, microstructured polymer environments using both 2-photon polymerisation and soft lithography techniques. Specifically, we have designed and fabricated biodegradable microwell-shaped devices using an in house synthetized polymer (4-arm photocurable poly-lactid acid) on a bespoke 2-photon polymerisation (2PP) set-up. We have studied swelling and degradation of the constructs as well as biocompatibility. Moreover, we have explored the potential of these constructs as artificial neural cell substrates by culturing NG108-15 cells (mouse neuroblastoma; rat glioma hybrid) and human neural progenitor cells on the microstructures. Finally, we have studied the effects of our artificial microenvironments upon neurite length and cell density. Springer Science+Business Media New York, 2015 2 photon-polymerisation (2PP) nationallicence Neural microdevice nationallicence Biodegradable nationallicence Stem cell microenvironment nationallicence Gill Andrew Biomaterials and Tissue Engineering Group, Department of Materials Science and Engineering, Kroto Research Institute, University of Sheffield, Broad Lane, S3 7HQ, Sheffield, UK aut Ortega Ílida Bioengineering and Health Technologies Group, The School of Clinical Dentistry, University of Sheffield Claremont Crescent, S10 2 TA, Sheffield, UK aut Kelly Stephen New Jersey Neuroscience Institute at JFK Medical Center, 65 James Street, Edison, New Jersey, USA aut Claeyssens Frederik Biomaterials and Tissue Engineering Group, Department of Materials Science and Engineering, Kroto Research Institute, University of Sheffield, Broad Lane, S3 7HQ, Sheffield, UK aut Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-10 1387-2176 17:2<1 2015 17 10544 https://doi.org/10.1007/s10544-015-9929-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10544-015-9929-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gill Andrew Biomaterials and Tissue Engineering Group, Department of Materials Science and Engineering, Kroto Research Institute, University of Sheffield, Broad Lane, S3 7HQ, Sheffield, UK aut NATIONALLICENCE 700 1- Ortega Ílida Bioengineering and Health Technologies Group, The School of Clinical Dentistry, University of Sheffield Claremont Crescent, S10 2 TA, Sheffield, UK aut NATIONALLICENCE 700 1- Kelly Stephen New Jersey Neuroscience Institute at JFK Medical Center, 65 James Street, Edison, New Jersey, USA aut NATIONALLICENCE 700 1- Claeyssens Frederik Biomaterials and Tissue Engineering Group, Department of Materials Science and Engineering, Kroto Research Institute, University of Sheffield, Broad Lane, S3 7HQ, Sheffield, UK aut NATIONALLICENCE 773 0- Biomedical Microdevices Springer US; http://www.springer-ny.com 17/2(2015-04-01), 1-10 1387-2176 17:2<1 2015 17 10544