Alginate core-shell beads for simplified three-dimensional tumor spheroid culture and drug screening
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| 024 | 7 | 0 | |a 10.1007/s10544-014-9918-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10544-014-9918-5 | ||
| 245 | 0 | 0 | |a Alginate core-shell beads for simplified three-dimensional tumor spheroid culture and drug screening |h [Elektronische Daten] |c [Linfen Yu, Cynthia Ni, Samantha Grist, Carmen Bayly, Karen Cheung] |
| 520 | 3 | |a We demonstrate that when using cell-laden core-shell hydrogel beads to support the generation of tumor spheroids, the shell structure reduces the out-of-bead and monolayer cell proliferation that occurs during long-term culture of tumor cells within core-only alginate beads. We fabricate core-shell beads in a two-step process using simple, one-layer microfluidic devices. Tumor cells encapsulated within the bead core will proliferate to form multicellular aggregates which can serve as three-dimensional (3-D) models of tumors in drug screening. Encapsulation in an alginate shell increased the time that cells could be maintained in three-dimensional culture for MCF-7 breast cancer cells prior to out-of-bead proliferation, permitting formation of spheroids over a period of 14 days without the need move the cell-laden beads to clean culture flasks to separate beads from underlying monolayers. Tamoxifen and docetaxel dose response shows decreased toxicity for multicellular aggregates in three-dimensional core-shell bead culture compared to monolayer. Using simple core-only beads gives mixed monolayer and 3-D culture during drug screening, and alters the treatment result compared to the 3-D core-shell or the 2-D monolayer groups, as measured by standard proliferation assay. By preventing the out-of-bead proliferation and subsequent monolayer formation that is observed with core-only beads, the core-shell structure can obviate the requirement to transfer the beads to a new culture flask during drug screening, an important consideration for cell-based drug screening and drugs which have high multicellular resistance index. | |
| 540 | |a Springer Science+Business Media New York, 2015 | ||
| 690 | 7 | |a Microfluidics |2 nationallicence | |
| 690 | 7 | |a 3-D cell culture |2 nationallicence | |
| 690 | 7 | |a Cell-laden hydrogel beads |2 nationallicence | |
| 690 | 7 | |a Drug screening |2 nationallicence | |
| 700 | 1 | |a Yu |D Linfen |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | |
| 700 | 1 | |a Ni |D Cynthia |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | |
| 700 | 1 | |a Grist |D Samantha |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | |
| 700 | 1 | |a Bayly |D Carmen |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | |
| 700 | 1 | |a Cheung |D Karen |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | |
| 773 | 0 | |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-9 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10544-014-9918-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10544-014-9918-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Linfen |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ni |D Cynthia |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Grist |D Samantha |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bayly |D Carmen |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cheung |D Karen |u Department of Electrical and Computer Engineering, University of British Columbia, 2332 Main Mall, V6T 1Z4, Vancouver, Canada |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Biomedical Microdevices |d Springer US; http://www.springer-ny.com |g 17/2(2015-04-01), 1-9 |x 1387-2176 |q 17:2<1 |1 2015 |2 17 |o 10544 | ||