caa a22 4500 605480540 CHVBK 20210128100416.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11099-015-0084-7 doi (NATIONALLICENCE)springer-10.1007/s11099-015-0084-7 Effects of low temperature and low irradiance on the physiological characteristics and related gene expression of different pepper species [Elektronische Daten] [L. Ou, G. Wei, Z. Zhang, X. Dai, X. Zou] Pepper is a thermophilous and heliophilic vegetable. In China, pepper is grown in greenhouse during winter and spring under lower temperature and irradiation. In this study, we investigated the effects of low temperature and low irradiance (LTLI) on the physiological characteristics and the expression of related genes in five pepper species, Capsicum annuum L. (CA), C. baccatum L. (CB), C. chinense Jacquin (CC), C. frutescens L. (CF), and C. pubescens Ruiz & Pavon (CP) in order to screen for greenhouse species that is resistant to such adverse conditions. We observed significant reductions not only in photosynthetic pigments and stomatal conductance but also in proline, total soluble sugar, enzyme activity, and root activity; disordered arrangements of leaf palisade and spongy tissues; and first rising and then falling expression of C-repeat binding factor (CBF3) and cold-regulated genes (CORc410). These results indicate that pepper is not resistant to LTLI. We also found that CP showed significantly higher photosynthetic activity, more proline and total soluble sugar, higher enzyme activity, higher root activity, higher CBF3 and CORc410 expression levels, more tightly packed leaf palisade and spongy tissues, and thicker bundle sheath than the other four species did under LTLI, while CF exhibited the lowest values for these indicators. It demonstrated significant differences in the ability to resist to LTLI among different species, with CP showing the strongest resistance, followed by CB. Therefore, we recommend the introduction of CP and CB to greenhouse cultivation to further screen for low temperature and low light-resistant pepper varieties to increase pepper production by strengthening intervariety hybridization. The Institute of Experimental Botany, 2015 adventitious root nationallicence catalase nationallicence chlorophyll content nationallicence osmotic substances nationallicence photosynthesis nationallicence superoxide dismutase nationallicence CA : Capsicum annuum L nationallicence Car : carotenoids nationallicence CAT : catalase nationallicence CB : Capsicum baccatum L nationallicence CBF3 : C-repeat binding factor nationallicence CC : Capsicum chinense Jacquin nationallicence CF : Capsicum frutescens L nationallicence Chl : chlorophyll nationallicence CORc410 : cold-regulated gene nationallicence CP : Capsicum pubescens Ruiz & Pavon nationallicence E : transpiration rate nationallicence Fm : maximal fluorescence nationallicence Fm′ : maximal fluorescence under light nationallicence FM : fresh mass nationallicence F0 : minimal initial fluorescence nationallicence F0′ : minimal fluorescence nationallicence g s : stomatal conductance nationallicence GR : glutathione reductase nationallicence LTLI : low temperature and low irradiance nationallicence qN : nonphotochemical quenching nationallicence qP : photochemical quenching nationallicence P N : net photosynthetic rate nationallicence SOD : superoxide dismutase nationallicence POD : peroxidase nationallicence WUE : water-use efficiency nationallicence ΦPSII : actual photochemical efficiency of PSII in the light nationallicence Ou L. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut Wei G. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut Zhang Z. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut Dai X. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut Zou X. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/1(2015-03-01), 85-94 0300-3604 53:1<85 2015 53 11099 https://doi.org/10.1007/s11099-015-0084-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0084-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ou L. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut NATIONALLICENCE 700 1- Wei G. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut NATIONALLICENCE 700 1- Zhang Z. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut NATIONALLICENCE 700 1- Dai X. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut NATIONALLICENCE 700 1- Zou X. Vegetable Institution of Hunan Academy of Agricultural Science, 410125, Hunan Changsha, China aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/1(2015-03-01), 85-94 0300-3604 53:1<85 2015 53 11099