caa a22 4500 605480613 CHVBK 20210128100416.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s11099-015-0095-4 doi (NATIONALLICENCE)springer-10.1007/s11099-015-0095-4 A marine phytoplankton Prymnesium parvum upregulates the component proteins of photosystem II under iron stress [Elektronische Daten] [M. Rahman, M. Rahman, T. Maki, T. Nishiuchi, T. Asano, H. Hasegawa] Iron is an essential limiting factor for primary production in many marine systems. The present study investigated differential regulation of protein expression in marine phytoplankton Prymnesium parvum under low Fe concentration. The phytoplankton was grown in f/2 culture medium in artificial seawater with low (0.0025 μM) and high (0.05 μM) Fe concentrations. Two-dimensional differential gel electrophoresis and matrix-assisted laser desorption-ionization-time of flight-mass spectrometer analysis were performed for protein identification and characterization. The growth of the alga declined substantially under the low Fe compared to the high Fe concentration. Under low Fe conditions, P. parvum upregulated 10 proteins including chloroplastic ATP synthase subunit b, D2 protein of PSII, D1 protein of PSII reaction centre, and light harvesting complex II protein, most of which are associated with photosynthetic activities in PSII. The results suggest that the marine alga P. parvum altered the biosynthesis of several photosynthetic proteins in order to cope with low Fe conditions. The Institute of Experimental Botany, 2015 Fe limitation nationallicence growth rate nationallicence photosynthesis nationallicence protein expression nationallicence Prymnesium parvum nationallicence 2-D DIGE : 2-dimensional differential gel electrophoresis nationallicence Chl : chlorophyll nationallicence HF : high Fe concentration nationallicence IEF : isoelectric focusing nationallicence LF : low Fe concentration nationallicence MALDI-TOF-MS : matrix-assisted laser desorption-ionization-time of flight-mass spectrometer nationallicence NCBI : National Center for Biotechnology Information nationallicence OD : optical density nationallicence OEE : oxygen-evolving enhancer nationallicence PBS : phosphate buffer saline nationallicence PQ : plastoquinon nationallicence ROS : reactive oxygen species nationallicence Rahman M. Graduate School of Natural Science and Technology, Kanazawa University, 920-1192, Kakuma, Kanazawa, Japan aut Rahman M. Centre for Environmental Sustainability, School of the Environment, University of Technology Sydney, PO Box 123, 2007, Broadway, NSW, Australia aut Maki T. Institute of Science and Engineering, Kanazawa University, 920-1192, Kakuma, Kanazawa, Japan aut Nishiuchi T. Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 920-0934, Takaramachi, Kanazawa, Japan aut Asano T. Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 920-0934, Takaramachi, Kanazawa, Japan aut Hasegawa H. Institute of Science and Engineering, Kanazawa University, 920-1192, Kakuma, Kanazawa, Japan aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/1(2015-03-01), 136-143 0300-3604 53:1<136 2015 53 11099 https://doi.org/10.1007/s11099-015-0095-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0095-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rahman M. Graduate School of Natural Science and Technology, Kanazawa University, 920-1192, Kakuma, Kanazawa, Japan aut NATIONALLICENCE 700 1- Rahman M. Centre for Environmental Sustainability, School of the Environment, University of Technology Sydney, PO Box 123, 2007, Broadway, NSW, Australia aut NATIONALLICENCE 700 1- Maki T. Institute of Science and Engineering, Kanazawa University, 920-1192, Kakuma, Kanazawa, Japan aut NATIONALLICENCE 700 1- Nishiuchi T. Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 920-0934, Takaramachi, Kanazawa, Japan aut NATIONALLICENCE 700 1- Asano T. Division of Functional Genomics, Advanced Science Research Center, Kanazawa University, 920-0934, Takaramachi, Kanazawa, Japan aut NATIONALLICENCE 700 1- Hasegawa H. Institute of Science and Engineering, Kanazawa University, 920-1192, Kakuma, Kanazawa, Japan aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/1(2015-03-01), 136-143 0300-3604 53:1<136 2015 53 11099