caa a22 4500 605480680 CHVBK 20210128100416.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11099-015-0111-8 doi (NATIONALLICENCE)springer-10.1007/s11099-015-0111-8 Overexpression of maize phospho enol pyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane [Elektronische Daten] [W. Shen, G. Chen, J. Xu, Y. Jiang, L. Liu, Z. Gao, J. Ma, X. Chen, T. Chen, C. Lv] Drought impacts severely crop photosynthesis and productivity. Development of transgenic rice overexpressing maize phosphoenolpyruvate carboxylase (PEPC) is a promising strategy for improving crop production under drought stress. However, the molecular mechanisms of protection from PEPC are not yet clear. The objective of this study was: first, to characterize the response of individual photosynthetic components to drought stress; second, to study the physiological and molecular mechanisms underlying the drought tolerance of transgenic rice (cv. Kitaake) over-expressing maize PEPC. Our results showed that PEPC overexpressing improved the ability of transgenic rice to conserve water and pigments during drying as compared to wild type. Despite the fact that drought induced reactive oxygen species and damaged photosystems (especially, PSI) in both lines, higher intercellular CO2 concentration protected the photosynthetic complexes, peptides, and also ultrastructure of thylakoid membranes against the oxidative damage in transgenic rice. In conclusion, although photosynthetic apparatus suffered an inevitable and asymmetric impairment during drought conditions, PEPC effectively alleviated the oxidative damage on photosystems and enhanced the drought tolerance by increasing intercellular CO2 concentration. Our investigation provided critical clues for exploring the feasibility of using C4 photosynthesis to increase the yield of rice under the aggravated global warming. The Institute of Experimental Botany, 2015 drought stress nationallicence phospho enol pyruvate carboxylase nationallicence transgenic rice nationallicence oxidative stress nationallicence BN-PAGE : blue native polyacrylamide gel electrophoresis nationallicence Chl : chlorophyll nationallicence C i : intercellular CO2 concentration nationallicence DM : dry mass nationallicence DS : drought stress nationallicence FM : fresh mass nationallicence g s : stomatal conductance nationallicence Ft : fast chlorophyll a fluorescence transients nationallicence MDA : malondialdehyde nationallicence OEC : oxygen evolving complex nationallicence O2 ·− : superoxide anion nationallicence PEPC : phosphoenolpyruvate carboxylase nationallicence P N : net photosynthetic rate nationallicence RC : PSII reaction center nationallicence Rf : relative mobility nationallicence ROS : reactive oxygen species nationallicence RWC : relative water content nationallicence T-PEPC : transgenic rice overexpressing PEPC nationallicence TM : turgid mass nationallicence WT : wild type nationallicence Shen W. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut Chen G. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut Xu J. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut Jiang Y. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut Liu L. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut Gao Z. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut Ma J. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut Chen X. University of Illinois at Urbana Champaign, 61801, Urbana, IL, USA aut Chen T. Fu Ren High School, Biological Base of Jiangsu Province, Huiyou Road 1, 214123, Wuxi, China aut Lv C. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/3(2015-09-01), 436-446 0300-3604 53:3<436 2015 53 11099 https://doi.org/10.1007/s11099-015-0111-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0111-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen W. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut NATIONALLICENCE 700 1- Chen G. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut NATIONALLICENCE 700 1- Xu J. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut NATIONALLICENCE 700 1- Jiang Y. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut NATIONALLICENCE 700 1- Liu L. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut NATIONALLICENCE 700 1- Gao Z. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut NATIONALLICENCE 700 1- Ma J. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut NATIONALLICENCE 700 1- Chen X. University of Illinois at Urbana Champaign, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Chen T. Fu Ren High School, Biological Base of Jiangsu Province, Huiyou Road 1, 214123, Wuxi, China aut NATIONALLICENCE 700 1- Lv C. College of Life Sciences, Nanjing Normal University, Wenyuan Road 1, 210023, Nanjing, China aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/3(2015-09-01), 436-446 0300-3604 53:3<436 2015 53 11099