caa a22 4500 605480699 CHVBK 20210128100417.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11099-015-0107-4 doi (NATIONALLICENCE)springer-10.1007/s11099-015-0107-4 Effects of Ca2+ and polyethylene glycol on the chlorophyll fluorescence parameters of transgenic OsCaS rice ( Oryza sativa L.) [Elektronische Daten] [R. Wei, Y. Liu, Y. Sui, M. Xu, S. Liu, X. Zhao] Ca2+ is an important factor mediating many biotic and abiotic stress responses in plants. In this study, we measured the chlorophyll (Chl) fluorescence of transgenic rice with increased or decreased expression of a calcium-sensing receptor (OsCaS) gene during water deficit caused by polyethylene glycol to prove our hypothesis that increased Ca2+ in combination with increased OsCaS could enhance the drought resistance of transgenic rice. Transcript abundance (evaluated by RT-PCR) was significantly lower in OsCaS antisense line 766 (AS766) than that in the wild type, while the overexpression line 777 (O777) showed four times higher amount than that in the wild type. Chl fluorescence showed that the photochemical quantum yield of PSII in the light increased due to addition of Ca2+ in the O777, but dropped in the AS766. Nonphotochemical quenching increased under stress in both transgenic lines and in the wild type, but less in the O777. Nonregulatory quantum yield of energy dissipation showed no significant change under drought stress. Photochemical quenching was significantly higher in the O777 than those in the AS766 and in the wild type after the Ca2+ treatment. In the absence of stress, the electron transport rate (ETR) was significantly higher in the O777 than in both the AS766 and the wild type. In contrast, the ETR of the wild type and both transgenic lines decreased under drought stress, while the effect of polyethylene glycol was partially alleviated by Ca2+ addition in the O777. In summary, excitation energy conversion and dissipation by PSII were regulated by Ca2+ in the O777. It might partially alleviate the effect of drought stress, whereas addition of Ca2+ had no effect in the wild type and the AS766. The Institute of Experimental Botany, 2015 calcium-sensing receptor nationallicence fluorescence kinetic curves nationallicence gene expression nationallicence photosynthesis nationallicence AS766 : OsCaS antisense line 766 nationallicence CaS : calcium-sensing receptor nationallicence [Ca2+] : concentration of Ca2+ nationallicence [Ca2+]i : concentration of cytosolic Ca2+ nationallicence [Ca2+]o : concentration of extracellular Ca2+ nationallicence ETR : electron transport rate nationallicence O777 OsCaS : overexpression line 777 nationallicence PEG : polyethylene glycol nationallicence qP : photochemical quenching nationallicence WT : wild type nationallicence YNO : nonregulatory quantum yield of energy dissipation nationallicence YNPQ : nonphotochemical quenching nationallicence YPSII : practical quantum yield of PSII nationallicence Wei R. College of Life Science, Capital Normal University, 100048, Beijing, China aut Liu Y. College of Life Science, Capital Normal University, 100048, Beijing, China aut Sui Y. College of Life Science, Capital Normal University, 100048, Beijing, China aut Xu M. College of Life Science, Capital Normal University, 100048, Beijing, China aut Liu S. College of Life Science, Capital Normal University, 100048, Beijing, China aut Zhao X. College of Life Science, Capital Normal University, 100048, Beijing, China aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/3(2015-09-01), 336-341 0300-3604 53:3<336 2015 53 11099 https://doi.org/10.1007/s11099-015-0107-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0107-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei R. College of Life Science, Capital Normal University, 100048, Beijing, China aut NATIONALLICENCE 700 1- Liu Y. College of Life Science, Capital Normal University, 100048, Beijing, China aut NATIONALLICENCE 700 1- Sui Y. College of Life Science, Capital Normal University, 100048, Beijing, China aut NATIONALLICENCE 700 1- Xu M. College of Life Science, Capital Normal University, 100048, Beijing, China aut NATIONALLICENCE 700 1- Liu S. College of Life Science, Capital Normal University, 100048, Beijing, China aut NATIONALLICENCE 700 1- Zhao X. College of Life Science, Capital Normal University, 100048, Beijing, China aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/3(2015-09-01), 336-341 0300-3604 53:3<336 2015 53 11099