caa a22 4500 605480737 CHVBK 20210128100417.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11099-015-0129-y doi (NATIONALLICENCE)springer-10.1007/s11099-015-0129-y Structural and functional changes in the photosynthetic apparatus of Chlamydomonas reinhardtii during nitrogen deprivation and replenishment [Elektronische Daten] [É. Preininger, A. Kósa, Z. Lőrincz, P. Nyitrai, J. Simon, B. Böddi, Á. Keresztes, I. Gyurján] Nitrogen is an essential factor for normal plant and algal development. As a component of nucleic acids, proteins, and chlorophyll (Chl) molecules, it has a crucial role in the organization of a functioning photosynthetic apparatus. Our aim was to study the effects of nitrogen starvation in cultures of the unicellular green alga, Chlamydomonas reinhardtii, maintained on nitrogen-free, and then on nitrogen-containing medium. During the three-week-long degreening process, considerable changes were observed in the Chl content, the ratio of Chl-protein complexes, and photosynthetic activity of the cultures as well as in the ultrastructure of single chloroplasts. The regreening process was much faster then the degradation; total greening of the cells occurred within four days. The rate of regeneration depended on the nitrogen content. At least 50% of the normal nitrogen content of Tris-Acetate-Phosphate (TAP) medium was required in the medium for the complete regreening of the cells and regeneration of chloroplasts. The Institute of Experimental Botany, 2015 electron microscopy nationallicence nitrogen starvation nationallicence O2 evolution nationallicence 77K fluorescence nationallicence Fv/Fm : maximal quantum yield of PSII photochemistry nationallicence Chl : chlorophyll nationallicence CP43 and CP47 : core complex proteins of PSII nationallicence DG : degreening process nationallicence P680 : primary electron donor of PSII nationallicence RG : regreening process nationallicence TAP : Tris-Acetate-Phosphate nationallicence Preininger É. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut Kósa A. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut Lőrincz Z. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut Nyitrai P. Department of Plant Physiology and Molecular Plant Biology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut Simon J. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut Böddi B. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut Keresztes Á. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut Gyurján I. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/3(2015-09-01), 369-377 0300-3604 53:3<369 2015 53 11099 https://doi.org/10.1007/s11099-015-0129-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0129-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Preininger É. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Kósa A. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Lőrincz Z. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Nyitrai P. Department of Plant Physiology and Molecular Plant Biology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Simon J. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Böddi B. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Keresztes Á. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut NATIONALLICENCE 700 1- Gyurján I. Department of Plant Anatomy, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117, Budapest, Hungary aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/3(2015-09-01), 369-377 0300-3604 53:3<369 2015 53 11099