caa a22 4500 605480915 CHVBK 20210128100418.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11099-015-0126-1 doi (NATIONALLICENCE)springer-10.1007/s11099-015-0126-1 Response of photosynthesis to short-term drought stress in rice seedlings overexpressing C4 phospho enol pyruvate carboxylase from maize and millet [Elektronische Daten] [Z. Ding, X. Sun, S. Huang, B. Zhou, M. Zhao] Abiotic stresses induce phosphoenolpyruvate carboxylase (PEPC) expression in C3 plants which suggests PEPC function in plant adaptation to stresses. Here, we studied the response of photosynthesis to short-term drought stress in rice seedlings overexpressing C4 PEPC from maize and millet. The transgenic lines exhibited 1.2-5.5 fold of PEPC activities than the wild type before the treatment, while 1.5-8.5 fold after five or ten days of water deficit. Net photosynthetic rate (P N) declined less during the water stress and recovered more after rewatering in the transgenic lines. These changes were accompanied with changes in the stomatal conductance (g s). The lower decrease in P N and g s resulted in significantly higher intrinsic water use efficiency in the transgenic rice lines after ten days of water withdrawal. There were no significant differences between the wild type and transgenic lines in maximum photochemical efficiency of PSII and photochemical quenching. The nonphotochemical quenching and the quantum efficiency of PSII maintained both higher in transgenic lines than those in the wild type during drought stress. This indicated that the transgenic lines could dissipate more excess energy to heat to protect PSII. Our result suggested that the increased PEPC activities in rice could alleviate the decrease of photosynthesis during short-term drought stress. The Institute of Experimental Botany, 2015 chlorophyll nationallicence chlorophyll fluorescence nationallicence gas exchange nationallicence photoprotection nationallicence stomatal opening nationallicence C i : intercellular CO2 concentration nationallicence E : transpiration rate nationallicence Fv/Fm : maximum photochemical efficiency of PSII nationallicence g s : stomatal conductance nationallicence NPQ : nonphotochemical quenching nationallicence PEPC : phosphoenolpyruvate carboxylase nationallicence P N : net photosynthetic rate nationallicence qP : photochemical quenching coefficient nationallicence WT : wild type nationallicence WUE : water-use efficiency (= PN/E) nationallicence ΦPSII : quantum efficiency of PSII nationallicence ZM07 : rice line No. 7 transformed with the intact PEPC gene from Zea mays nationallicence ZM30 : rice line No. 30 transformed with the intact PEPC gene from Zea mays nationallicence PRM25 : rice line No. 25 transformed with PEPC gene from Setaria italica under the control of rice Rubisco small subunit promoter nationallicence PRM38 : rice line No. 38 transformed with PEPC gene from Setaria italica under the control of rice Rubisco small subunit promoter nationallicence Ding Z. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut Sun X. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut Huang S. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut Zhou B. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut Zhao M. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/4(2015-12-01), 481-488 0300-3604 53:4<481 2015 53 11099 https://doi.org/10.1007/s11099-015-0126-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0126-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ding Z. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut NATIONALLICENCE 700 1- Sun X. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut NATIONALLICENCE 700 1- Huang S. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut NATIONALLICENCE 700 1- Zhou B. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut NATIONALLICENCE 700 1- Zhao M. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Physiology and Ecology, Ministry of Agriculture, 100081, Beijing, China aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/4(2015-12-01), 481-488 0300-3604 53:4<481 2015 53 11099