caa a22 4500 605480931 CHVBK 20210128100418.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11099-015-0128-z doi (NATIONALLICENCE)springer-10.1007/s11099-015-0128-z Proteomic study reveals photosynthesis as downstream of both MAP kinase and cAMP signaling pathways in Chlamydomonas reinhardtii [Elektronische Daten] [C. Lee, J. Rhee, D. Kim, Y. Choi] Previously, our data indicated that both cAMP and MAP kinase signaling play important roles in microalgal physiology as well as in lipid or carotenoid biosynthesis. In order to understand downstream genes of these signaling pathways, we employed proteomics approach. Both signal pathways were first altered with specific signaling inhibitors or modulators. Treatment of specific inhibitors changed microalgal size and increased lipid contents. With the microalgal cells after treatments of specific signaling inhibitor or modulators, we performed the proteomics analysis to identify downstream genes responsible for these phenotypes. Interestingly, multiple photosynthesis genes were identified, particularly proteins associated with PSII. Our data suggested that MAP kinase and cAMP signaling affect the photosynthesis, thereby leading to microalgal lipid or carotenoid biosynthesis. The Institute of Experimental Botany, 2015 gene regulation nationallicence mass spectrometry nationallicence two-dimensional gel electrophoresis nationallicence cAMP : cyclic adenosine monophosphate nationallicence Car : carotenoid nationallicence DE : decrease nationallicence IBMX : 3-isobutyl-1-methylxanthine nationallicence IN : increase nationallicence MAP kinase : mitogen-activated protein kinase nationallicence MEK : MAP kinase kinase or MAP 2K nationallicence MEKK : MAP kinase kinase kinase or MAP 3K nationallicence OT : only in treated nationallicence OU : only in untreated nationallicence 2-DE : two-dimensional gel electrophoresis nationallicence Lee C. Department of Bioprocess Engineering, Chonbuk National University, 561-756, Jeonju, Korea aut Rhee J. Department of Food Science and Engineering, Ewha Womans University, 03760, Seoul, Korea aut Kim D. Department of Bioprocess Engineering, Chonbuk National University, 561-756, Jeonju, Korea aut Choi Y. Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 136-713, Seoul, Korea aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/4(2015-12-01), 625-629 0300-3604 53:4<625 2015 53 11099 https://doi.org/10.1007/s11099-015-0128-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0128-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee C. Department of Bioprocess Engineering, Chonbuk National University, 561-756, Jeonju, Korea aut NATIONALLICENCE 700 1- Rhee J. Department of Food Science and Engineering, Ewha Womans University, 03760, Seoul, Korea aut NATIONALLICENCE 700 1- Kim D. Department of Bioprocess Engineering, Chonbuk National University, 561-756, Jeonju, Korea aut NATIONALLICENCE 700 1- Choi Y. Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, 136-713, Seoul, Korea aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/4(2015-12-01), 625-629 0300-3604 53:4<625 2015 53 11099