caa a22 4500 605481172 CHVBK 20210128100419.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11099-015-0104-7 doi (NATIONALLICENCE)springer-10.1007/s11099-015-0104-7 Photosynthetic activities, C3 and C4 indicative enzymes and the role of photoperiod in dormancy induction in ‘Chunjie' peach [Elektronische Daten] [H. Zhang, D. Li, Q. Tan, H. Gao, D. Gao] Our study examined the relationship between photosynthetic performance and activities of key photosynthetic enzymes to understand the photosynthetic variation and reasons for the variation during dormancy induction under different photoperiods in peach (Prunus persica L. cv. Chunjie). Furthermore, the study explained the changes in the key enzymes from the viewpoint of differential proteomics. The results showed that the leaf net photosynthetic rate (P N) and stomatal conductance tended to decrease, while the intercellular CO2 concentration rose, which indicated that the reduced P N resulted from nonstomatal limitation. During the dormancy induction period, the activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) declined, which was the main reason for the reduced P N. Two-dimensional electrophoresis maps and differential protein identification demonstrated that the decrease in activity of the photosynthetic enzymes was mainly due to enzymatic degradation. The enzyme degradation by a long-day treatment occurred later and to a lesser degree than that of the short-day treatment. In the long-day treatment, the carboxylation activity of Rubisco was higher than that of the control treatment, and the PEPC activity and the ratio of the PEPC/Rubisco activity were lower than the corresponding activities during the control treatment. These differences under long-day conditions were significant but did not occur in the short-day treatment, suggesting that the C4 pathway might be more active under short-day conditions. The Institute of Experimental Botany, 2015 gas exchange nationallicence MALDI-TOF nationallicence nonstomatal limitation nationallicence Rubisco large subunit nationallicence two-dimensional electrophoresis nationallicence C i : intercellular CO2 concentration nationallicence CK : control nationallicence g s : stomatal conductance nationallicence IEF : isoelectric focusing nationallicence IPG : immobilized pH gradient strips nationallicence LD : long day nationallicence MALDI-TOF : matrix-assisted laser desorption/ionization time of flight nationallicence Mr : relative molecular mass nationallicence MS : mass spectrometry nationallicence PEP : phosphoenolpyruvate nationallicence PEPC : phosphoenolpyruvate carboxylase nationallicence PI : isoelectric point nationallicence PMF : peptide mass fingerprinting nationallicence P N : net photosynthetic rate nationallicence SD : short day nationallicence 2-DE : two-dimensional electrophoresis nationallicence Zhang H. College of Life Sciences, Postdoctoral Station of Biology, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut Li D. College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut Tan Q. College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut Gao H. College of Life Sciences, Postdoctoral Station of Biology, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut Gao D. College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/2(2015-06-01), 269-278 0300-3604 53:2<269 2015 53 11099 https://doi.org/10.1007/s11099-015-0104-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0104-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang H. College of Life Sciences, Postdoctoral Station of Biology, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut NATIONALLICENCE 700 1- Li D. College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut NATIONALLICENCE 700 1- Tan Q. College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut NATIONALLICENCE 700 1- Gao H. College of Life Sciences, Postdoctoral Station of Biology, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut NATIONALLICENCE 700 1- Gao D. College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, 271018, Tai'an, China aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/2(2015-06-01), 269-278 0300-3604 53:2<269 2015 53 11099