caa a22 4500 605481180 CHVBK 20210128100419.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11099-015-0102-9 doi (NATIONALLICENCE)springer-10.1007/s11099-015-0102-9 Ultraviolet-B radiation or heat cause changes in photosynthesis, antioxidant enzyme activities and pollen performance in olive tree [Elektronische Daten] [G. Koubouris, N. Kavroulakis, I. Metzidakis, M. Vasilakakis, A. Sofo] The present study attempts to determine how some physiological and reproductive functions of olive tree (Olea europaea L., cv. Koroneiki) respond to enhanced UV-B radiation or heat. Enhanced UV-B radiation was applied to (1) three-year-old potted plants in an open nursery (corresponded to ca. 16% ozone depletion), and (2) in vitro cultured pollen samples (220 μmol m−2 s−1, PAR = 400−700 nm + UV-B at 7.5, 15.0, or 22.5 kJ m−2 d−1). Potted olive plants were also subjected to high temperature (38 ± 4°C) for 28 h to mimic heat levels regularly measured in olive growing areas. A significant effect of UV-B on photosynthetic rate was observed. However, enhanced UV-B radiation did affect neither chlorophyll nor carotenoid content, supporting previous reports on hardiness of the photosynthetic apparatus in olive. Increased superoxide dismutase activity was observed in UV-B-treated olive plants (+ 225%), whereas no effect was found in the plants under heat stress. Neither UV-B and nor heat did affect H2O2 accumulation in the plant tissues. However, the same treatments resulted in enhanced lipid peroxidation (+ 18% for UV-B and + 15% for heat), which is likely linked to other reactive oxygen species. The increased guaiacol peroxidase activity observed in both treatments (+ 32% for UV-B and + 49% for heat) is related to the defense against oxidative membrane damage. The observed reduction in pollen germination (20-39%) and tube length (11-44%) could have serious implications on olive yields, especially for low fruit-setting cultivars or in years and environments with additional unfavorable conditions. UV-B and heat effects described here support the hypothesis that plant response to a given stressor is affected by the overall context and that a holistic approach is necessary to determine plant strategies for climate change adaptation. The Institute of Experimental Botany, 2015 abiotic stress nationallicence climate change nationallicence lipid peroxidation nationallicence ozone nationallicence pollen germination nationallicence ANOVA : analysis of variance nationallicence Car : carotenoids nationallicence Chl : chlorophyll nationallicence C i : intercellular CO2 concentration nationallicence DM : dry mass nationallicence FM : fresh mass nationallicence GPOX : guaiacol peroxidase nationallicence g s : stomatal conductance nationallicence MDA : malondialdehyde nationallicence P N : net photosynthetic rate nationallicence ROS : reactive oxygen species nationallicence SOD : superoxide dismutase nationallicence TBARS : thiobarbituric acid reactive substances nationallicence UV-B : ultraviolet-B radiation nationallicence Koubouris G. Institute for Olive Tree and Subtropical Plants, National Agricultural Research Foundation (N.AG.RE.F.), ELGO ‘DEMETER' Agrokipio, 73100, Chania, Greece aut Kavroulakis N. Institute for Olive Tree and Subtropical Plants, National Agricultural Research Foundation (N.AG.RE.F.), ELGO ‘DEMETER' Agrokipio, 73100, Chania, Greece aut Metzidakis I. Institute for Olive Tree and Subtropical Plants, National Agricultural Research Foundation (N.AG.RE.F.), ELGO ‘DEMETER' Agrokipio, 73100, Chania, Greece aut Vasilakakis M. School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut Sofo A. School of Agricultural, Forestry, Food and Environmental Sciences, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/2(2015-06-01), 279-287 0300-3604 53:2<279 2015 53 11099 https://doi.org/10.1007/s11099-015-0102-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0102-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koubouris G. Institute for Olive Tree and Subtropical Plants, National Agricultural Research Foundation (N.AG.RE.F.), ELGO ‘DEMETER' Agrokipio, 73100, Chania, Greece aut NATIONALLICENCE 700 1- Kavroulakis N. Institute for Olive Tree and Subtropical Plants, National Agricultural Research Foundation (N.AG.RE.F.), ELGO ‘DEMETER' Agrokipio, 73100, Chania, Greece aut NATIONALLICENCE 700 1- Metzidakis I. Institute for Olive Tree and Subtropical Plants, National Agricultural Research Foundation (N.AG.RE.F.), ELGO ‘DEMETER' Agrokipio, 73100, Chania, Greece aut NATIONALLICENCE 700 1- Vasilakakis M. School of Agriculture, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece aut NATIONALLICENCE 700 1- Sofo A. School of Agricultural, Forestry, Food and Environmental Sciences, Università degli Studi della Basilicata, Via dell'Ateneo Lucano 10, 85100, Potenza, Italy aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/2(2015-06-01), 279-287 0300-3604 53:2<279 2015 53 11099