caa a22 4500 605481199 CHVBK 20210128100419.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11099-015-0097-2 doi (NATIONALLICENCE)springer-10.1007/s11099-015-0097-2 Extracellular ATP affects chlorophyll fluorescence of kidney bean ( Phaseolus vulgaris ) leaves through Ca2+ and H2O2-dependent mechanism [Elektronische Daten] [H. Feng, Q. Jiao, K. Sun, L. Jia, W. Tian] Extracellular ATP (eATP) has been considered as an important extracellular compound to mediate several physiological processes in plant cells. We investigated the effects of eATP on chlorophyll (Chl) fluorescence characteristics of kidney bean (Phaseolus vulgaris) leaves. Treatment with exogenous ATP at 1 mM showed no significant effect on the maximal photochemical efficiency of PSII. However, the treatment significantly enhanced the values of the PSII operating efficiency (ΦPSII), rate of photosynthetic electron transport through PSII (ETR), and photochemical quenching (qP), while the values of the nonphotochemical quenching (qN) and quantum yield of regulated energy dissipation of PSII (YNPQ) significantly decreased. Our observations indicated that eATP stimulated the PSII photochemistry in kidney bean leaves. Similarly, the treatment with exogenous Ca2+ or H2O2 at 1 mM caused also the significant increase in ΦPSII, qP, and ETR and the significant decrease in qN and YNPQ. LaCl3 (an inhibitor of Ca2+ channels) and dimethylthiourea (a scavenger of H2O2) abolished the effects of exogenous ATP. The results suggest that the role of eATP in enhancing the PSII photochemistry could be related to a Ca2+ or H2O2 signaling pathway. The Institute of Experimental Botany, 2015 photosynthesis nationallicence reactive oxygen species nationallicence signaling molecules nationallicence DMTU : dimethylthiourea nationallicence eATP : extracellular ATP nationallicence ETR : the rate of photosynthetic electron transport through PSII nationallicence Fv/Fm : the maximal photochemical efficiency of PSII nationallicence qN : the nonphotochemical quenching nationallicence qP : the photochemical quenching nationallicence ROS : reactive oxygen species nationallicence YNO : the quantum yield of nonregulated energy dissipation of PSII nationallicence YNPQ : the quantum yield of regulated energy dissipation of PSII nationallicence ΦPSII : the PSII operating efficiency nationallicence Feng H. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut Jiao Q. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut Sun K. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut Jia L. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut Tian W. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/2(2015-06-01), 201-206 0300-3604 53:2<201 2015 53 11099 https://doi.org/10.1007/s11099-015-0097-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11099-015-0097-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feng H. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut NATIONALLICENCE 700 1- Jiao Q. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut NATIONALLICENCE 700 1- Sun K. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut NATIONALLICENCE 700 1- Jia L. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut NATIONALLICENCE 700 1- Tian W. College of Life Science, Northwest Normal University, 730070, Lanzhou, China aut NATIONALLICENCE 773 0- Photosynthetica The Institute of Experimental Biology of the Czech Academy of Sciences 53/2(2015-06-01), 201-206 0300-3604 53:2<201 2015 53 11099