caa a22 4500 605490457 CHVBK 20210128100505.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10147-014-0680-8 doi (NATIONALLICENCE)springer-10.1007/s10147-014-0680-8 Cytokine profile in cervical mucosa of Japanese patients with cervical intraepithelial neoplasia [Elektronische Daten] [Takashi Iwata, Takuma Fujii, Kenji Morii, Miyuki Saito, Juri Sugiyama, Hiroshi Nishio, Tohru Morisada, Kyoko Tanaka, Tomonori Yaguchi, Yutaka Kawakami, Daisuke Aoki] Background: Immune responses in the uterine cervix are considered to play an important role in persistent human papillomavirus (HPV) infection and carcinogenesis, but many aspects of the mechanism are still unclear. The goal of this study was to measure cytokines to analyze immune responses in patients with cervical intraepithelial neoplasia (CIN). Materials and methods: The levels of 17 cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, G-CSF, GM-CSF, INF-γ, MCP-1, MIP-1β, and TNFα) in cervical mucus were simultaneously measured using a multiplex immunoassay in 52 high-grade squamous intraepithelial lesion (HSIL) cases and overproduction of IL-1β, IL-8, and MIP-1β was identified. The levels of these 3 cytokines were measured in 130 patients with or without CIN lesions using enzyme-linked immunosorbent assay. The associations of the cytokine levels with the cytology, infecting HPV type, and status of cigarette smoking were investigated. Results: IL-1β and IL-8 levels were associated with the cytology, and these levels were higher in HSIL cases than in NILM (negative for intraepithelial lesion and malignancy) and LSIL (low-grade squamous intraepithelial lesion) cases (P=0.005, P=0.001, respectively). The MIP-1β level was significantly lower in smokers (P=0.018) and high-risk (HR)-HPV-infected patients (P=0.021). Conclusions: Enhanced expression of IL-1β and IL-8 indicates that Th2 inflammatory responses become stronger in the local uterine cervical region with the progression of CIN lesions, and a decrease in the MIP-1β level may be advantageous for immunoescape of HPV. Cigarette smoking may further facilitate persistent HPV infection. Japan Society of Clinical Oncology, 2014 CIN nationallicence Cytokine nationallicence Cytology nationallicence HPV nationallicence Cigarette smoking nationallicence Iwata Takashi Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut Fujii Takuma Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut Morii Kenji Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan aut Saito Miyuki Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut Sugiyama Juri Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut Nishio Hiroshi Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut Morisada Tohru Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut Tanaka Kyoko Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut Yaguchi Tomonori Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan aut Kawakami Yutaka Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan aut Aoki Daisuke Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut International Journal of Clinical Oncology Springer Japan 20/1(2015-02-01), 126-133 1341-9625 20:1<126 2015 20 10147 https://doi.org/10.1007/s10147-014-0680-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10147-014-0680-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iwata Takashi Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Fujii Takuma Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Morii Kenji Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Saito Miyuki Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Sugiyama Juri Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Nishio Hiroshi Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Morisada Tohru Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Tanaka Kyoko Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Yaguchi Tomonori Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Kawakami Yutaka Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan aut NATIONALLICENCE 700 1- Aoki Daisuke Department of Obstetrics and Gynecology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- International Journal of Clinical Oncology Springer Japan 20/1(2015-02-01), 126-133 1341-9625 20:1<126 2015 20 10147