caa a22 4500 605490589 CHVBK 20210128100505.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10147-015-0848-x doi (NATIONALLICENCE)springer-10.1007/s10147-015-0848-x Clinical significance of hypoxia-inducible factor 1 and VEGF-A in osteosarcoma [Elektronische Daten] [Huanli Zhao, Yuhui Wu, Yang Chen, Hongmei Liu] Background: Although the function of hypoxia-inducible factor 1 (HIF1) in many kinds of solid tumor has been revealed, the significance of HIF1 in osteosarcoma is still controversial and not well understood. Methods: Immunohistochemistry was used to detect HIF1 expression. The correlation between HIF1 and clinicopathology factors was analyzed by use of chi-squared tests. The prognostic value of HIF1 was evaluated by univariate and multivariate analysis. Moreover, the function of HIF1 in osteosarcoma cells was further investigated in in-vitro experiments by regulating HIF1 and vascular endothelial growth factor-A (VEGF-A) expression. Results: Expression of HIF1 was high for 56.82% of the samples in our investigation. HIF1 expression was significantly associated with positive metastasis (P=0.037). By use of the Kaplan-Meier method, high expression of HIF1 was proved to be related to poorer overall survival (P=0.007). By use of a Cox-regression model, HIF1 was identified as an independent prognostic biomarker (P=0.019). We also proved that HIF1 can promote osteosarcoma invasion in hypoxia by inducing VEGF-A expression. Conclusions: HIF1 was identified as an independent prognostic biomarker in osteosarcoma. It can promote osteosarcoma cell invasion by inducing VEGF-A expression, indicating that HIF1 is a potential drug target in osteosarcoma. Japan Society of Clinical Oncology, 2015 Hypoxia-inducible factor 1 nationallicence Osteosarcoma nationallicence VEGF-A nationallicence Prognosis nationallicence Invasion nationallicence Zhao Huanli Department of Orthopedics, Linyi People's Hospital, 276003, Linyi, China aut Wu Yuhui Department of Orthopedics, Linyi People's Hospital, 276003, Linyi, China aut Chen Yang Department of Orthopedics, Linyi People's Hospital, 276003, Linyi, China aut Liu Hongmei Department of Surgery, Linyi People's Hospital, 276003, Linyi, Shangdong Province, China aut International Journal of Clinical Oncology Springer Japan 20/6(2015-12-01), 1233-1243 1341-9625 20:6<1233 2015 20 10147 https://doi.org/10.1007/s10147-015-0848-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10147-015-0848-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Huanli Department of Orthopedics, Linyi People's Hospital, 276003, Linyi, China aut NATIONALLICENCE 700 1- Wu Yuhui Department of Orthopedics, Linyi People's Hospital, 276003, Linyi, China aut NATIONALLICENCE 700 1- Chen Yang Department of Orthopedics, Linyi People's Hospital, 276003, Linyi, China aut NATIONALLICENCE 700 1- Liu Hongmei Department of Surgery, Linyi People's Hospital, 276003, Linyi, Shangdong Province, China aut NATIONALLICENCE 773 0- International Journal of Clinical Oncology Springer Japan 20/6(2015-12-01), 1233-1243 1341-9625 20:6<1233 2015 20 10147