caa a22 4500 605491259 CHVBK 20210128100509.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10147-015-0782-y doi (NATIONALLICENCE)springer-10.1007/s10147-015-0782-y Expression and role of V1A subunit of V-ATPases in gastric cancer cells [Elektronische Daten] [Pengfei Liu, Haijiao Chen, Lanxiu Han, Xiaoping Zou, Weidong Shen] Background: Vacuolar-ATPases (V-ATPases) play an important role in maintaining a relatively neutral pHi (internal pH) and are responsible for the progression of cancer. V-ATPases contain different subunits and few studies have been conducted on subunit V1A. This study aimed to investigate the gene expression of V1A subunit of V-ATPases in gastric cancer tissues and explore its role in the progression and prognosis of gastric cancer. Methods: The protein expressions of the V1A subunit of V-ATPase gene in 100 normal gastric specimens and 100 gastric cancer tissues were determined by immunohistochemistry. The role of V1A subunit of V-ATPases was studied using a specific small interfering RNA (siRNA). Results: The positive expression rate of the V1A subunit of V-ATPases was 76% in gastric cancer tissue samples, much higher than that in normal tissue samples (30%, P<0.05), and was correlated with histological grade (P=0.001), lymph node metastasis (P=0.002), TNM (P=0.040), and vascular invasion (P=0.010), but not with patient age, sex, depth of tumor invasion, tumor size, or histological type. The median overall survival times of 76 patients who had positive staining for tumor cell V1A subunit of V-ATPases and 24 patients who had negative staining were 31.7 and 59.2months, respectively. When the expression of V1A subunit was knocked down using siRNA, the proliferation and invasion of gastric cancer cells in vitro were significantly inhibited. Conclusions: V1A subunit of V-ATPases can be a prognostic indicator for poor outcome and is a therapeutic target in gastric cancer. Japan Society of Clinical Oncology, 2015 V-ATPases nationallicence Gastric cancer nationallicence Expression nationallicence Liu Pengfei Department of Gastroenterology, The Jiangyin People's Hospital, Medical School of University of Southeast, 214400, Wuxi, People's Republic of China aut Chen Haijiao Department of Gastroenterology, The Jiangyin People's Hospital, Medical School of University of Southeast, 214400, Wuxi, People's Republic of China aut Han Lanxiu Department of Gastroenterology, The Jiangyin People's Hospital, Medical School of University of Southeast, 214400, Wuxi, People's Republic of China aut Zou Xiaoping Department of Gastroenterology, Nanjing Drum Tower Hospital Clinical College of Nanjing University, 210008, Nanjing, People's Republic of China aut Shen Weidong Department of Gastroenterology, The Jiangyin People's Hospital, Medical School of University of Southeast, 214400, Wuxi, People's Republic of China aut International Journal of Clinical Oncology Springer Japan 20/4(2015-08-01), 725-735 1341-9625 20:4<725 2015 20 10147 https://doi.org/10.1007/s10147-015-0782-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10147-015-0782-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Pengfei Department of Gastroenterology, The Jiangyin People's Hospital, Medical School of University of Southeast, 214400, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Chen Haijiao Department of Gastroenterology, The Jiangyin People's Hospital, Medical School of University of Southeast, 214400, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Han Lanxiu Department of Gastroenterology, The Jiangyin People's Hospital, Medical School of University of Southeast, 214400, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Zou Xiaoping Department of Gastroenterology, Nanjing Drum Tower Hospital Clinical College of Nanjing University, 210008, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Shen Weidong Department of Gastroenterology, The Jiangyin People's Hospital, Medical School of University of Southeast, 214400, Wuxi, People's Republic of China aut NATIONALLICENCE 773 0- International Journal of Clinical Oncology Springer Japan 20/4(2015-08-01), 725-735 1341-9625 20:4<725 2015 20 10147