Increased levels of sperm protein 17 mRNA and circulating antibodies in periampullary carcinoma patients
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| 024 | 7 | 0 | |a 10.1007/s10147-014-0762-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s10147-014-0762-7 | ||
| 245 | 0 | 0 | |a Increased levels of sperm protein 17 mRNA and circulating antibodies in periampullary carcinoma patients |h [Elektronische Daten] |c [Shivam Singh, Anoop Saraya, Rinu Sharma] |
| 520 | 3 | |a Background: Present-day diagnostic modalities for detecting periampullary carcinoma are suboptimal, and currently used proven markers lack specificity and sensitivity. Methods: In order to assess the diagnostic potential of sperm protein 17, a cancer testis antigen, quantitative real-time PCR was performed to evaluate the expression of sperm protein 17 in tissue and sera specimens collected from periampullary carcinoma patients and normal subjects. Additionally, circulating levels of anti-sperm protein 17 antibodies were determined in sera of periampullary carcinoma patients and normal subjects using ELISA. Results: Aberrant expression of sperm protein 17 was found in 14/15 (93%) periampullary cancer tissues when compared with distant matched nonmalignant tissues (P=0.006, Mann-Whitney U test). None of the distant matched nonmalignant tissues showed increased expression of sperm protein 17 mRNA. Area under the curve, sensitivity, and specificity were 0.791, 87, and 73%, respectively. Increased levels of sperm protein 17 mRNA were demonstrated in sera of periampullary carcinoma patients (P=0.020, Student's t test). Circulating levels of anti-sperm protein 17 antibody were found to be significantly elevated in 27/30 (90%) periampullary carcinoma patients (P<0.001, Student's t test). Area under the curve, sensitivity, and specificity were 0.954, 86.7, and 96.3%, respectively. Only two of the normal subjects (7%) showed elevated levels of anti-sperm protein 17 antibody. Conclusion: For the first time, our findings suggest that high levels of sperm protein 17 mRNA as well as increased circulating anti-sperm protein 17 antibodies can be used to distinguish periampullary cancer patients from healthy individuals, highlighting the diagnostic potential of sperm protein 17. | |
| 540 | |a Japan Society of Clinical Oncology, 2014 | ||
| 690 | 7 | |a Sp17 |2 nationallicence | |
| 690 | 7 | |a Periampullary carcinoma |2 nationallicence | |
| 690 | 7 | |a Real-time PCR |2 nationallicence | |
| 690 | 7 | |a ELISA |2 nationallicence | |
| 690 | 7 | |a Sera |2 nationallicence | |
| 700 | 1 | |a Singh |D Shivam |u University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector-16(c), Dwarka, 110078, New Delhi, India |4 aut | |
| 700 | 1 | |a Saraya |D Anoop |u Department of Gastroenterology, All India Institute of Medical Sciences, Ansari Nagar, 110029, New Delhi, India |4 aut | |
| 700 | 1 | |a Sharma |D Rinu |u University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector-16(c), Dwarka, 110078, New Delhi, India |4 aut | |
| 773 | 0 | |t International Journal of Clinical Oncology |d Springer Japan |g 20/4(2015-08-01), 736-744 |x 1341-9625 |q 20:4<736 |1 2015 |2 20 |o 10147 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s10147-014-0762-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s10147-014-0762-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Singh |D Shivam |u University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector-16(c), Dwarka, 110078, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Saraya |D Anoop |u Department of Gastroenterology, All India Institute of Medical Sciences, Ansari Nagar, 110029, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sharma |D Rinu |u University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector-16(c), Dwarka, 110078, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t International Journal of Clinical Oncology |d Springer Japan |g 20/4(2015-08-01), 736-744 |x 1341-9625 |q 20:4<736 |1 2015 |2 20 |o 10147 | ||