caa a22 4500 605491364 CHVBK 20210128100509.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10147-014-0762-7 doi (NATIONALLICENCE)springer-10.1007/s10147-014-0762-7 Increased levels of sperm protein 17 mRNA and circulating antibodies in periampullary carcinoma patients [Elektronische Daten] [Shivam Singh, Anoop Saraya, Rinu Sharma] Background: Present-day diagnostic modalities for detecting periampullary carcinoma are suboptimal, and currently used proven markers lack specificity and sensitivity. Methods: In order to assess the diagnostic potential of sperm protein 17, a cancer testis antigen, quantitative real-time PCR was performed to evaluate the expression of sperm protein 17 in tissue and sera specimens collected from periampullary carcinoma patients and normal subjects. Additionally, circulating levels of anti-sperm protein 17 antibodies were determined in sera of periampullary carcinoma patients and normal subjects using ELISA. Results: Aberrant expression of sperm protein 17 was found in 14/15 (93%) periampullary cancer tissues when compared with distant matched nonmalignant tissues (P=0.006, Mann-Whitney U test). None of the distant matched nonmalignant tissues showed increased expression of sperm protein 17 mRNA. Area under the curve, sensitivity, and specificity were 0.791, 87, and 73%, respectively. Increased levels of sperm protein 17 mRNA were demonstrated in sera of periampullary carcinoma patients (P=0.020, Student's t test). Circulating levels of anti-sperm protein 17 antibody were found to be significantly elevated in 27/30 (90%) periampullary carcinoma patients (P<0.001, Student's t test). Area under the curve, sensitivity, and specificity were 0.954, 86.7, and 96.3%, respectively. Only two of the normal subjects (7%) showed elevated levels of anti-sperm protein 17 antibody. Conclusion: For the first time, our findings suggest that high levels of sperm protein 17 mRNA as well as increased circulating anti-sperm protein 17 antibodies can be used to distinguish periampullary cancer patients from healthy individuals, highlighting the diagnostic potential of sperm protein 17. Japan Society of Clinical Oncology, 2014 Sp17 nationallicence Periampullary carcinoma nationallicence Real-time PCR nationallicence ELISA nationallicence Sera nationallicence Singh Shivam University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector-16(c), Dwarka, 110078, New Delhi, India aut Saraya Anoop Department of Gastroenterology, All India Institute of Medical Sciences, Ansari Nagar, 110029, New Delhi, India aut Sharma Rinu University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector-16(c), Dwarka, 110078, New Delhi, India aut International Journal of Clinical Oncology Springer Japan 20/4(2015-08-01), 736-744 1341-9625 20:4<736 2015 20 10147 https://doi.org/10.1007/s10147-014-0762-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10147-014-0762-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh Shivam University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector-16(c), Dwarka, 110078, New Delhi, India aut NATIONALLICENCE 700 1- Saraya Anoop Department of Gastroenterology, All India Institute of Medical Sciences, Ansari Nagar, 110029, New Delhi, India aut NATIONALLICENCE 700 1- Sharma Rinu University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sector-16(c), Dwarka, 110078, New Delhi, India aut NATIONALLICENCE 773 0- International Journal of Clinical Oncology Springer Japan 20/4(2015-08-01), 736-744 1341-9625 20:4<736 2015 20 10147