caa a22 4500 605491437 CHVBK 20210128100510.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10147-015-0797-4 doi (NATIONALLICENCE)springer-10.1007/s10147-015-0797-4 FOXP3 autoantibody as a potential early prognostic serum biomarker in patients with cervical cancer [Elektronische Daten] [Shuang Xu, Mingmei Huangfu, Xueli Jia, Xiaowei Song, Baosheng Sun, Kuang-Hui Lee, Linlin Liu, Shilong Sun] Background: Overexpression of tumor-associated antigens has been reported in many types of cancer and may trigger secretion of their autoantibodies. The present work was designed to test whether circulating antibody to FOXP3 protein-derived antigens was altered in early cervical cancer and cervical benign tumors. Methods: A total of 141 patients with cervical cancer, 133 patients with cervical benign tumors and 148 healthy age-matched volunteers were recruited. The level of circulating anti-FOXP3 IgG antibody was tested using an enzyme-linked immunosorbent assay developed in-house with linear peptide antigens derived from FOXP3 protein. The linear peptide antigens were designed according to the computational prediction of HLA-II epitopes. Results: Student's t test showed that anti-FOXP3 IgG in the malignant tumor group and the benign tumor group was significantly higher than in the control group (t=6.127, p<0.001; t=2.704, p=0.007). In addition, patients with stage I cervical cancer (t=2.968, p=0.003) had a significantly higher level of FOXP3 autoantibodies than patients with benign tumors. The sensitivity against >90% specificity was 20.6% with an interassay deviation of 11.7% in the cervical cancer group. Based on a cut-off value determined by the 98th percentile of the control group IgG levels, the anti-FOXP3 IgG positivity was 2.1% in patients with cervical cancer compared to 2.0% in the health controls (chi-squared=0.004, p=0.952, OR=1.051, 95% CI 0.209-5.295). Conclusion: The circulating autoantibody to FOXP3 reflecting the continuous development of the cervical lesion, may be a potential biomarker with early prognostic values for cervical cancer. Japan Society of Clinical Oncology, 2015 Autoantibody nationallicence Biomarkers nationallicence Cervical cancer nationallicence FOXP3 nationallicence Tumor immunity nationallicence Xu Shuang Department of Radiotherapy, Second Hospital of Jilin University, 130041, Changchun, China aut Huangfu Mingmei Department of Radiotherapy, Second Hospital of Jilin University, 130041, Changchun, China aut Jia Xueli Department of Radiotherapy, Second Hospital of Jilin University, 130041, Changchun, China aut Song Xiaowei Ministry of Health Key Laboratory of Radiobiology, Jilin University, 130021, Changchun, China aut Sun Baosheng Department of Radiotherapy, Tumor Hospital of Jilin Province, 130012, Changchun, China aut Lee Kuang-Hui Pei-Ling Guan-Si Hospital, Hsin-Fu Kuansi Township, 30647, Hsinchu County, Taiwan aut Liu Linlin Department of Radiotherapy, Second Hospital of Jilin University, 130041, Changchun, China aut Sun Shilong Ministry of Health Key Laboratory of Radiobiology, Jilin University, 130021, Changchun, China aut International Journal of Clinical Oncology Springer Japan 20/5(2015-10-01), 982-988 1341-9625 20:5<982 2015 20 10147 https://doi.org/10.1007/s10147-015-0797-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10147-015-0797-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Shuang Department of Radiotherapy, Second Hospital of Jilin University, 130041, Changchun, China aut NATIONALLICENCE 700 1- Huangfu Mingmei Department of Radiotherapy, Second Hospital of Jilin University, 130041, Changchun, China aut NATIONALLICENCE 700 1- Jia Xueli Department of Radiotherapy, Second Hospital of Jilin University, 130041, Changchun, China aut NATIONALLICENCE 700 1- Song Xiaowei Ministry of Health Key Laboratory of Radiobiology, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Sun Baosheng Department of Radiotherapy, Tumor Hospital of Jilin Province, 130012, Changchun, China aut NATIONALLICENCE 700 1- Lee Kuang-Hui Pei-Ling Guan-Si Hospital, Hsin-Fu Kuansi Township, 30647, Hsinchu County, Taiwan aut NATIONALLICENCE 700 1- Liu Linlin Department of Radiotherapy, Second Hospital of Jilin University, 130041, Changchun, China aut NATIONALLICENCE 700 1- Sun Shilong Ministry of Health Key Laboratory of Radiobiology, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 773 0- International Journal of Clinical Oncology Springer Japan 20/5(2015-10-01), 982-988 1341-9625 20:5<982 2015 20 10147