caa a22 4500 605491674 CHVBK 20210128100511.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10147-015-0789-4 doi (NATIONALLICENCE)springer-10.1007/s10147-015-0789-4 HPV genotyping for triage of women with abnormal cervical cancer screening results: a multicenter prospective study [Elektronische Daten] [Yuko Nakamura, Koji Matsumoto, Toyomi Satoh, Ken Nishide, Akiko Nozue, Koji Shimabukuro, Seiichi Endo, Kimihiro Nagai, Akinori Oki, Hiroyuki Ochi, Yukio Morishita, Masayuki Noguchi, Hiroyuki Yoshikawa] Background: In cervical cancer screening programs, women with abnormal cytology are referred for colposcopy for histological evaluation. We examined whether a human papillomavirus (HPV) genotyping assay could be used to identify women who do not need immediate colposcopy and biopsy because of low risk of cervical intraepithelial neoplasia grade 3 or worse (CIN3+). Methods: We prospectively evaluated test performance for 2 carcinogenic HPV genotypes (HPV16/18), for 8 types (HPV16/18/31/33/35/45/52/58), and for 13 types (HPV16/18/31/33/35/45/51/52/56/58/59/68) for prediction of histological CIN3+results among 427 screen-positive women referred for colposcopy. The study subjects consisted of 214 women with low-grade squamous intraepithelial lesion (LSIL), 184 with high-grade squamous intraepithelial lesion (HSIL), and 29 with atypical squamous cells, cannot exclude HSIL (ASC-H). Results: Among women with LSIL cytology, HPV16/18 positivity was 29.4% and increased to 58.9% for 8 types and to 74.8% for 13 types (P<0.001). The risk of CIN3+biopsy results was still 7.9% for women testing negative for HPV16/18, but decreased to 0.0% for those testing negative for at least eight types of HPV (HPV16/18/31/33/35/45/52/58). Although HPV genotyping results enabled additional risk stratification among women with HSIL/ASC-H cytology, the risk of histological CIN3+diagnosis among women testing negative for eight types or more was still sufficiently high (>35%) to warrant immediate colposcopy referral. Conclusions: Of women with LSIL cytology, those testing negative for at least eight of the highest-risk types of HPV (HPV16/18/31/33/35/45/52/58) may not need immediate colposcopy and biopsy. This would reduce the number of colposcopy referrals by approximately 40%. However, the HPV genotyping assay is not likely to alter the clinical management of women with HSIL/ASC-H. Japan Society of Clinical Oncology, 2015 Human papillomavirus nationallicence Genotyping nationallicence Colposcopy nationallicence Cervical intraepithelial neoplasia nationallicence Cervical cancer nationallicence Low-grade squamous intraepithelial lesion nationallicence Nakamura Yuko Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut Matsumoto Koji Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut Satoh Toyomi Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut Nishide Ken Department of Gynecology, Tsukuba Medical Center Hospital, 305-8558, Tsukuba, Japan aut Nozue Akiko Department of Gynecology, Tsukuba Medical Center Hospital, 305-8558, Tsukuba, Japan aut Shimabukuro Koji Department of Obstetrics and Gynecology, Tsuchiura Kyodo General Hospital, 300-0053, Tsuchiura, Japan aut Endo Seiichi Department of Obstetrics and Gynecology, Tsuchiura Kyodo General Hospital, 300-0053, Tsuchiura, Japan aut Nagai Kimihiro Department of Gynecology, Ibaraki Prefectural Central Hospital, 309-1793, Kasama, Japan aut Oki Akinori Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut Ochi Hiroyuki Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut Morishita Yukio Department of Pathology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut Noguchi Masayuki Department of Pathology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut Yoshikawa Hiroyuki Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut International Journal of Clinical Oncology Springer Japan 20/5(2015-10-01), 974-981 1341-9625 20:5<974 2015 20 10147 https://doi.org/10.1007/s10147-015-0789-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10147-015-0789-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Yuko Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut NATIONALLICENCE 700 1- Matsumoto Koji Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut NATIONALLICENCE 700 1- Satoh Toyomi Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut NATIONALLICENCE 700 1- Nishide Ken Department of Gynecology, Tsukuba Medical Center Hospital, 305-8558, Tsukuba, Japan aut NATIONALLICENCE 700 1- Nozue Akiko Department of Gynecology, Tsukuba Medical Center Hospital, 305-8558, Tsukuba, Japan aut NATIONALLICENCE 700 1- Shimabukuro Koji Department of Obstetrics and Gynecology, Tsuchiura Kyodo General Hospital, 300-0053, Tsuchiura, Japan aut NATIONALLICENCE 700 1- Endo Seiichi Department of Obstetrics and Gynecology, Tsuchiura Kyodo General Hospital, 300-0053, Tsuchiura, Japan aut NATIONALLICENCE 700 1- Nagai Kimihiro Department of Gynecology, Ibaraki Prefectural Central Hospital, 309-1793, Kasama, Japan aut NATIONALLICENCE 700 1- Oki Akinori Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut NATIONALLICENCE 700 1- Ochi Hiroyuki Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut NATIONALLICENCE 700 1- Morishita Yukio Department of Pathology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut NATIONALLICENCE 700 1- Noguchi Masayuki Department of Pathology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut NATIONALLICENCE 700 1- Yoshikawa Hiroyuki Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, 305-8575, Tsukuba, Japan aut NATIONALLICENCE 773 0- International Journal of Clinical Oncology Springer Japan 20/5(2015-10-01), 974-981 1341-9625 20:5<974 2015 20 10147