caa a22 4500 605491720 CHVBK 20210128100511.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10147-014-0719-x doi (NATIONALLICENCE)springer-10.1007/s10147-014-0719-x The key role of calreticulin in immunomodulation induced by chemotherapeutic agents [Elektronische Daten] [Yoshiyuki Yamamura, Takahiro Tsuchikawa, Kengo Miyauchi, Shintaro Takeuchi, Masataka Wada, Toshihiko Kuwatani, Noriaki Kyogoku, Aki Kuroda, Takehiro Maki, Toshiaki Shichinohe, Satoshi Hirano] Background: It has recently been shown that certain chemotherapeutic agents can improve host immune responses. The present study aimed to demonstrate the mechanism by which chemotherapeutic agents modify the tumor microenvironment and induce tumor-specific immune responses. Methods: Three mouse cancer cell lines [CT26 mouse colon cancer cells, B16 melanoma cells and Lewis lung carcinoma (LLC)], 5 human carcinoma cell lines (human esophageal squamous cell carcinoma cell lines TE8 and HEC46 and the human pancreatic carcinoma cell lines PK-9, AsPC-1 and SUIT-2) and 5 chemotherapeutic agents [mitoxantrone (MIT), mitomycin C(MMC), 5-fluorouracil (5FU), camptothecin (CPT-11) and cisplatin (CDDP)] that are frequently used in a clinical setting for cancer treatment were utilized to investigate the surface expression level of calreticulin and HLA class I after exposure to chemotherapeutic agents. Results: Increased calreticulin (CRT) expression on the surface of mouse cell lines and, moreover, increased surface expression levels of both CRT and HLA class I in all human cell lineswere observed in cells treated by the chemotherapeutic agents as compared with non-treated cells. The surface expression level of CRT was significantly correlated with the HLA class I expression level in all human cell lines. Conclusions: In conclusion, chemotherapeutic drugs can improve the immunogenicity of cancer cells in a cell-specific manner through the mechanism of translocation of CRT. Japan Society of Clinical Oncology, 2014 Immunogenic cell death nationallicence Chemotherapy nationallicence Immunotherapy nationallicence Calreticulin nationallicence HLA class I nationallicence Yamamura Yoshiyuki Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Tsuchikawa Takahiro Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Miyauchi Kengo Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Takeuchi Shintaro Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Wada Masataka Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Kuwatani Toshihiko Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Kyogoku Noriaki Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Kuroda Aki Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Maki Takehiro Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Shichinohe Toshiaki Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut Hirano Satoshi Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut International Journal of Clinical Oncology Springer Japan 20/2(2015-04-01), 386-394 1341-9625 20:2<386 2015 20 10147 https://doi.org/10.1007/s10147-014-0719-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10147-014-0719-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamamura Yoshiyuki Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Tsuchikawa Takahiro Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Miyauchi Kengo Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Takeuchi Shintaro Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Wada Masataka Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Kuwatani Toshihiko Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Kyogoku Noriaki Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Kuroda Aki Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Maki Takehiro Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Shichinohe Toshiaki Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 700 1- Hirano Satoshi Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, N-15 W-17, 060-0815, Sapporo, Japan aut NATIONALLICENCE 773 0- International Journal of Clinical Oncology Springer Japan 20/2(2015-04-01), 386-394 1341-9625 20:2<386 2015 20 10147