caa a22 4500 605497494 CHVBK 20210128100541.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10441-015-9260-1 doi (NATIONALLICENCE)springer-10.1007/s10441-015-9260-1 In Silico Functional and Structural Characterization of H1N1 Influenza A Viruses Hemagglutinin, 2010-2013, Shiraz, Iran [Elektronische Daten] [Afagh Moattari, Behzad Dehghani, Nastaran Khodadad, Forogh Tavakoli] Hemagglutinin (HA) is a major virulence factor of influenza viruses and plays an important role in viral pathogenesis. Analysis of amino acid changes, epitopes' regions, glycosylation and phosphorylation sites have greatly contributed to the development of new generations of vaccine. The hemagglutinins of 10 selected isolates, 8 of 2010 and 2 of 2013 samples were sequenced and analyzed by several bioinformatic softwares and the results were compared with those of 3 vaccine isolates. The study detected several amino acid changes related to altered epitopes' sites, modification sites and physico-chemical properties. The results showed some conserved modification sites in HA structure. This study is the first analytical research on isolates obtained from Shiraz, Iran, and our results can be used to better understand the genetic diversity and antigenic variations in Iranian and Asian H1N1 pathogenic strains. Springer Science+Business Media Dordrecht, 2015 Influenza nationallicence Hemagglutinin nationallicence In silico nationallicence Glycosylation nationallicence Phosphorylation nationallicence Moattari Afagh Influenza Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, 71348-45794, Shiraz, Iran aut Dehghani Behzad Influenza Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, 71348-45794, Shiraz, Iran aut Khodadad Nastaran Influenza Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, 71348-45794, Shiraz, Iran aut Tavakoli Forogh Influenza Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, 71348-45794, Shiraz, Iran aut Acta Biotheoretica Springer Netherlands 63/2(2015-06-01), 183-202 0001-5342 63:2<183 2015 63 10441 https://doi.org/10.1007/s10441-015-9260-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10441-015-9260-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moattari Afagh Influenza Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, 71348-45794, Shiraz, Iran aut NATIONALLICENCE 700 1- Dehghani Behzad Influenza Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, 71348-45794, Shiraz, Iran aut NATIONALLICENCE 700 1- Khodadad Nastaran Influenza Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, 71348-45794, Shiraz, Iran aut NATIONALLICENCE 700 1- Tavakoli Forogh Influenza Research Center, Department of Bacteriology and Virology, Shiraz University of Medical Sciences, 71348-45794, Shiraz, Iran aut NATIONALLICENCE 773 0- Acta Biotheoretica Springer Netherlands 63/2(2015-06-01), 183-202 0001-5342 63:2<183 2015 63 10441