caa a22 4500 605497737 CHVBK 20210128100542.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6069-9 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6069-9 The consequence of an additional NADH dehydrogenase paralog on the growth of Gluconobacter oxydans DSM3504 [Elektronische Daten] [D. Kostner, B. Luchterhand, A. Junker, S. Volland, R. Daniel, J. Büchs, W. Liebl, A. Ehrenreich] Acetic acid bacteria such as Gluconobacter oxydans are used in several biotechnological processes due to their ability to perform rapid incomplete regio- and stereo-selective oxidations of a great variety of carbohydrates, alcohols, and related compounds by their membrane-bound dehydrogenases. In order to understand the growth physiology of industrial strains such as G. oxydans ATCC 621H that has high substrate oxidation rates but poor growth yields, we compared its genome sequence to the genome sequence of strain DSM 3504 that reaches an almost three times higher optical density. Although the genome sequences are very similar, DSM 3504 has additional copies of genes that are absent from ATCC 621H. Most importantly, strain DSM 3504 contains an additional type II NADH dehydrogenase (ndh) gene and an additional triosephosphate isomerase (tpi) gene. We deleted these additional paralogs from DSM 3504, overexpressed NADH dehydrogenase in ATCC 621H, and monitored biomass and the concentration of the representative cell components as well as O2 and CO2 transfer rates in growth experiments on mannitol. The data revealed a clear competition of membrane-bound dehydrogenases and NADH dehydrogenase for channeling electrons in the electron transport chain of Gluconobacter and an important role of the additional NADH dehydrogenase for increased growth yields. The less active the NADH dehydrogenase is, the more active is the membrane-bound polyol dehydrogenase. These results were confirmed by introducing additional ndh genes via plasmid pAJ78 in strain ATCC 621H, which leads to a marked increase of the growth rate. Springer-Verlag Berlin Heidelberg, 2014 Gluconobacter nationallicence Acetic acid bacteria nationallicence Growth nationallicence NADH dehydrogenase nationallicence Membrane-bound dehydrogenases nationallicence Electron transport chain nationallicence RAMOS nationallicence Kostner D. Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann Str. 4, 85354, Freising, Germany aut Luchterhand B. Department of Biochemical Engineering, RWTH Aachen University, Aachen, Germany aut Junker A. Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann Str. 4, 85354, Freising, Germany aut Volland S. Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University Göttingen, Göttingen, Germany aut Daniel R. Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University Göttingen, Göttingen, Germany aut Büchs J. Department of Biochemical Engineering, RWTH Aachen University, Aachen, Germany aut Liebl W. Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann Str. 4, 85354, Freising, Germany aut Ehrenreich A. Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann Str. 4, 85354, Freising, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 375-386 0175-7598 99:1<375 2015 99 253 https://doi.org/10.1007/s00253-014-6069-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6069-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kostner D. Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann Str. 4, 85354, Freising, Germany aut NATIONALLICENCE 700 1- Luchterhand B. Department of Biochemical Engineering, RWTH Aachen University, Aachen, Germany aut NATIONALLICENCE 700 1- Junker A. Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann Str. 4, 85354, Freising, Germany aut NATIONALLICENCE 700 1- Volland S. Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Daniel R. Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University Göttingen, Göttingen, Germany aut NATIONALLICENCE 700 1- Büchs J. Department of Biochemical Engineering, RWTH Aachen University, Aachen, Germany aut NATIONALLICENCE 700 1- Liebl W. Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann Str. 4, 85354, Freising, Germany aut NATIONALLICENCE 700 1- Ehrenreich A. Lehrstuhl für Mikrobiologie, Technische Universität München, Emil-Ramann Str. 4, 85354, Freising, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 375-386 0175-7598 99:1<375 2015 99 253