caa a22 4500 605497753 CHVBK 20210128100542.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6174-9 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6174-9 SUMO expression shortens the lag phase of Saccharomyces cerevisiae yeast growth caused by complex interactive effects of major mixed fermentation inhibitors found in hot-compressed water-treated lignocellulosic hydrolysate [Elektronische Daten] [Lahiru Jayakody, Masafumi Kadowaki, Keisuke Tsuge, Kenta Horie, Akihiro Suzuki, Nobuyuki Hayashi, Hiroshi Kitagaki] The complex inhibitory effects of inhibitors present in lignocellulose hydrolysate suppress the ethanol fermentation of Saccharomyces cerevisiae. Although the interactive inhibitory effects play important roles in the actual hydrolysate, few studies have investigated glycolaldehyde, the key inhibitor of hot-compressed water-treated lignocellulose hydrolysate. Given this challenge, we investigated the interactive effects of mixed fermentation inhibitors, including glycolaldehyde. First, we confirmed that glycolaldehyde was the most potent inhibitor in the hydrolysate and exerted interactive inhibitory effects in combination with major inhibitors. Next, through genome-wide analysis and megavariate data modeling, we identified SUMOylation as a novel potential mechanism to overcome the combinational inhibitory effects of fermentation inhibitors. Indeed, overall SUMOylation was increased and Pgk1, which produces an ATP molecule in glycolysis by substrate-level phosphorylation, was SUMOylated and degraded in response to glycolaldehyde. Augmenting the SUMO-dependent ubiquitin system in the ADH1-expressing strain significantly shortened the lag phase of growth, released cells from G2/M arrest, and improved energy status and glucose uptake in the inhibitor-containing medium. In summary, our study was the first to establish SUMOylation as a novel platform for regulating the lag phase caused by complex fermentation inhibitors. Springer-Verlag Berlin Heidelberg, 2014 SUMOylation nationallicence Yeast nationallicence Glycolaldehyde nationallicence Bioethanol nationallicence Jayakody Lahiru Department of Biochemistry and Applied Biosciences, United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, 890-8580, Kagoshima City, Kagoshima, Japan aut Kadowaki Masafumi Department of Environmental Sciences, Faculty of Agriculture, Saga University, 840-8502, Saga, Japan aut Tsuge Keisuke Industrial Technology Center of Saga, 114 Yaemizo, 849-0932, Saga, Japan aut Horie Kenta Department of Environmental Sciences, Faculty of Agriculture, Saga University, 840-8502, Saga, Japan aut Suzuki Akihiro Department of Biochemistry and Applied Biosciences, United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, 890-8580, Kagoshima City, Kagoshima, Japan aut Hayashi Nobuyuki Department of Biochemistry and Applied Biosciences, United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, 890-8580, Kagoshima City, Kagoshima, Japan aut Kitagaki Hiroshi Department of Biochemistry and Applied Biosciences, United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, 890-8580, Kagoshima City, Kagoshima, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 501-515 0175-7598 99:1<501 2015 99 253 https://doi.org/10.1007/s00253-014-6174-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6174-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jayakody Lahiru Department of Biochemistry and Applied Biosciences, United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, 890-8580, Kagoshima City, Kagoshima, Japan aut NATIONALLICENCE 700 1- Kadowaki Masafumi Department of Environmental Sciences, Faculty of Agriculture, Saga University, 840-8502, Saga, Japan aut NATIONALLICENCE 700 1- Tsuge Keisuke Industrial Technology Center of Saga, 114 Yaemizo, 849-0932, Saga, Japan aut NATIONALLICENCE 700 1- Horie Kenta Department of Environmental Sciences, Faculty of Agriculture, Saga University, 840-8502, Saga, Japan aut NATIONALLICENCE 700 1- Suzuki Akihiro Department of Biochemistry and Applied Biosciences, United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, 890-8580, Kagoshima City, Kagoshima, Japan aut NATIONALLICENCE 700 1- Hayashi Nobuyuki Department of Biochemistry and Applied Biosciences, United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, 890-8580, Kagoshima City, Kagoshima, Japan aut NATIONALLICENCE 700 1- Kitagaki Hiroshi Department of Biochemistry and Applied Biosciences, United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24, Korimoto, 890-8580, Kagoshima City, Kagoshima, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 501-515 0175-7598 99:1<501 2015 99 253