caa a22 4500 605497788 CHVBK 20210128100542.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-5821-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-5821-5 Biofilm dynamics characterization using a novel DO-MEA sensor: mass transport and biokinetics [Elektronische Daten] [Xavier Guimerà, Ana Moya, Antonio Dorado, Rosa Villa, David Gabriel, Gemma Gabriel, Xavier Gamisans] Biodegradation process modeling is an essential tool for the optimization of biotechnologies related to gaseous pollutant treatment. In these technologies, the predominant role of biofilm, particularly under conditions of no mass transfer limitations, results in a need to determine what processes are occurring within the same. By measuring the interior of the biofilms, an increased knowledge of mass transport and biodegradation processes may be attained. This information is useful in order to develop more reliable models that take biofilm heterogeneity into account. In this study, a new methodology, based on a novel dissolved oxygen (DO) and mass transport microelectronic array (MEA) sensor, is presented in order to characterize a biofilm. Utilizing the MEA sensor, designed to obtain DO and diffusivity profiles with a single measurement, it was possible to obtain distributions of oxygen diffusivity and biokinetic parameters along a biofilm grown in a flat plate bioreactor (FPB). The results obtained for oxygen diffusivity, estimated from oxygenation profiles and direct measurements, revealed that changes in its distribution were reduced when increasing the liquid flow rate. It was also possible to observe the effect of biofilm heterogeneity through biokinetic parameters, estimated using the DO profiles. Biokinetic parameters, including maximum specific growth rate, the Monod half-saturation coefficient of oxygen, and the maintenance coefficient for oxygen which showed a marked variation across the biofilm, suggest that a tool that considers the heterogeneity of biofilms is essential for the optimization of biotechnologies. Springer-Verlag Berlin Heidelberg, 2014 Heterotrophic biofilm nationallicence Dissolved oxygen nationallicence Biofilm profiling nationallicence MEA sensor nationallicence Effective diffusivity nationallicence Biokinetic parameters nationallicence Guimerà Xavier Department of Mining Engineering and Natural Resources, Universitat Politècnica de Catalunya, Avinguda de les Bases de Manresa 61-73, 08240, Manresa, Barcelona, Spain aut Moya Ana Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Esfera UAB, Campus Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut Dorado Antonio Department of Mining Engineering and Natural Resources, Universitat Politècnica de Catalunya, Avinguda de les Bases de Manresa 61-73, 08240, Manresa, Barcelona, Spain aut Villa Rosa Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Esfera UAB, Campus Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut Gabriel David Department of Chemical Engineering, Universitat Autònoma de Barcelona, Edifici Q, 08193, Bellaterra, Barcelona, Spain aut Gabriel Gemma Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Esfera UAB, Campus Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut Gamisans Xavier Department of Mining Engineering and Natural Resources, Universitat Politècnica de Catalunya, Avinguda de les Bases de Manresa 61-73, 08240, Manresa, Barcelona, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 55-66 0175-7598 99:1<55 2015 99 253 https://doi.org/10.1007/s00253-014-5821-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-5821-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guimerà Xavier Department of Mining Engineering and Natural Resources, Universitat Politècnica de Catalunya, Avinguda de les Bases de Manresa 61-73, 08240, Manresa, Barcelona, Spain aut NATIONALLICENCE 700 1- Moya Ana Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Esfera UAB, Campus Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut NATIONALLICENCE 700 1- Dorado Antonio Department of Mining Engineering and Natural Resources, Universitat Politècnica de Catalunya, Avinguda de les Bases de Manresa 61-73, 08240, Manresa, Barcelona, Spain aut NATIONALLICENCE 700 1- Villa Rosa Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Esfera UAB, Campus Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut NATIONALLICENCE 700 1- Gabriel David Department of Chemical Engineering, Universitat Autònoma de Barcelona, Edifici Q, 08193, Bellaterra, Barcelona, Spain aut NATIONALLICENCE 700 1- Gabriel Gemma Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Esfera UAB, Campus Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut NATIONALLICENCE 700 1- Gamisans Xavier Department of Mining Engineering and Natural Resources, Universitat Politècnica de Catalunya, Avinguda de les Bases de Manresa 61-73, 08240, Manresa, Barcelona, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 55-66 0175-7598 99:1<55 2015 99 253