caa a22 4500 605497842 CHVBK 20210128100543.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6031-x doi (NATIONALLICENCE)springer-10.1007/s00253-014-6031-x Vertical distribution of nitrite-dependent anaerobic methane-oxidising bacteria in natural freshwater wetland soils [Elektronische Daten] [Li-dong Shen, Qian Huang, Zhan-fei He, Xu Lian, Shuai Liu, Yun-feng He, Li-ping Lou, Xiang-yang Xu, Ping Zheng, Bao-lan Hu] Nitrite-dependent anaerobic methane oxidation (n-damo) is a recently discovered process that is catalysed by "Candidatus Methylomirabilis oxyfera”. In the present study, the vertical distribution (0-10, 20-30, 50-60 and 90-100cm) of M. oxyfera-like bacteria was investigated in Xiazhuhu wetland, the largest natural wetland on the southern Yangtze River (China). Phylogenetic analyses showed that group A of M. oxyfera-like bacteria and pmoA genes occurred primarily at depths of 50-60 and 90-100cm. Quantitative PCR further confirmed the presence of M. oxyfera-like bacteria in soil cores from different depths, with the highest abundance of 5.1 × 107 copiesg−1 dry soil at depth of 50-60cm. Stable isotope experiments demonstrated that the n-damo process occurred primarily at depths of 50-60 and 90-100cm, with the potential rates ranging from 0.2 to 14.5nmol CO2 g−1 dry soild−1. It was estimated that the methane flux may increase by approximately 2.7-4.3% in the examined wetland in the absence of n-damo. This study shows that the deep wetland soils (50-60 and 90-100cm) are the preferred habitats for M. oxyfera-like bacteria. The study also highlights the potential importance of these bacteria in the methane and nitrogen cycles in deep wetland soils. Springer-Verlag Berlin Heidelberg, 2014 n-damo nationallicence M. oxyfera nationallicence Vertical distribution nationallicence Methane cycle nationallicence Nitrogen cycle nationallicence Wetlands nationallicence Shen Li-dong Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Huang Qian Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut He Zhan-fei Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Lian Xu Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Liu Shuai Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut He Yun-feng Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Lou Li-ping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Xu Xiang-yang Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Zheng Ping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Hu Bao-lan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 349-357 0175-7598 99:1<349 2015 99 253 https://doi.org/10.1007/s00253-014-6031-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6031-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shen Li-dong Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Huang Qian Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- He Zhan-fei Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Lian Xu Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Liu Shuai Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- He Yun-feng Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Lou Li-ping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Xu Xiang-yang Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Zheng Ping Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Hu Bao-lan Department of Environmental Engineering, Zhejiang University, 310058, Hangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 349-357 0175-7598 99:1<349 2015 99 253