caa a22 4500 605497850 CHVBK 20210128100543.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6046-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6046-3 Inoculum selection is crucial to ensure operational stability in anaerobic digestion [Elektronische Daten] [Jo De Vrieze, Sylvia Gildemyn, Ramiro Vilchez-Vargas, Ruy Jáuregui, Dietmar Pieper, Willy Verstraete, Nico Boon] Anaerobic digestion is considered a key technology for the future bio-based economy. The microbial consortium carrying out the anaerobic digestion process is quite complex, and its exact role in terms of "elasticity”, i.e., the ability to rapidly adapt to changing conditions, is still unknown. In this study, the role of the initial microbial community in terms of operational stability and stress tolerance was evaluated during a 175-day experiment. Five different inocula from stable industrial anaerobic digesters were fed a mixture of waste activated sludge and glycerol. Increasing ammonium pulses were applied to evaluate stability and stress tolerance. A different response in terms of start-up and ammonium tolerance was observed among the different inocula. Methanosaetaceae were the dominant acetoclastic methanogens, yet, Methanosarcinaceae increased in abundance at elevated ammonium concentrations. A shift from a Firmicutes to a Proteobacteria dominated bacterial community was observed in failing digesters. Methane production was strongly positively correlated with Methanosaetaceae, but also with Bacteria related to Anaerolinaceae, Clostridiales, and Alphaproteobacteria. Volatile fatty acids were strongly positively correlated with Betaproteobacteria and Bacteroidetes, yet ammonium concentration only with Bacteroidetes. Overall, these results indicate the importance of inoculum selection to ensure stable operation and stress tolerance in anaerobic digestion. Springer-Verlag Berlin Heidelberg, 2014 Anaerobic digestion nationallicence Biogas nationallicence Microbial dynamics nationallicence Methanogenesis nationallicence Methanosaeta nationallicence De Vrieze Jo Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Gildemyn Sylvia Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Vilchez-Vargas Ramiro Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Jáuregui Ruy Microbial Interactions and Processes Research Group, Department of Medical Microbiology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany aut Pieper Dietmar Microbial Interactions and Processes Research Group, Department of Medical Microbiology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany aut Verstraete Willy Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Boon Nico Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 189-199 0175-7598 99:1<189 2015 99 253 https://doi.org/10.1007/s00253-014-6046-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6046-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Vrieze Jo Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Gildemyn Sylvia Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Vilchez-Vargas Ramiro Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Jáuregui Ruy Microbial Interactions and Processes Research Group, Department of Medical Microbiology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany aut NATIONALLICENCE 700 1- Pieper Dietmar Microbial Interactions and Processes Research Group, Department of Medical Microbiology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany aut NATIONALLICENCE 700 1- Verstraete Willy Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Boon Nico Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 189-199 0175-7598 99:1<189 2015 99 253