caa a22 4500 605497923 CHVBK 20210128100543.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-5688-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-5688-5 Kinetic and stoichiometric characterization of anoxic sulfide oxidation by SO-NR mixed cultures from anoxic biotrickling filters [Elektronische Daten] [Mabel Mora, Maikel Fernández, José Gómez, Domingo Cantero, Javier Lafuente, Xavier Gamisans, David Gabriel] Monitoring the biological activity in biotrickling filters is difficult since it implies estimating biomass concentration and its growth yield, which can hardly be measured in immobilized biomass systems. In this study, the characterization of a sulfide-oxidizing nitrate-reducing biomass obtained from an anoxic biotrickling filter was performed through the application of respirometric and titrimetric techniques. Previously, the biomass was maintained in a continuous stirred tank reactor under steady-state conditions resulting in a growth yield of 0.328 ± 0.045g VSS/gS. To properly assess biological activity in respirometric tests, abiotic assays were conducted to characterize the stripping of CO2 and sulfide. The global mass transfer coefficient for both processes was estimated. Subsequently, different respirometric tests were performed: (1) to solve the stoichiometry related to the autotrophic denitrification of sulfide using either nitrate or nitrite as electron acceptors, (2) to evaluate the inhibition caused by nitrite and sulfide on sulfide oxidation, and (3) to propose, calibrate, and validate a kinetic model considering both electron acceptors in the overall anoxic biodesulfurization process. The kinetic model considered a Haldane-type equation to describe sulfide and nitrite inhibitions, a non-competitive inhibition to reflect the effect of sulfide on the elemental sulfur oxidation besides single-step denitrification since no nitrite was produced during the biological assays. Springer-Verlag Berlin Heidelberg, 2014 Hydrogen sulfide nationallicence Biotrickling filter nationallicence SO-NR culture nationallicence Kinetics and stoichiometry nationallicence Respirometry nationallicence Titrimetry nationallicence Mora Mabel Department of Chemical Engineering, School of Engineering, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut Fernández Maikel Department of Chemical Engineering and Food Technologies, Faculty of Sciences, University of Cádiz, 11510, Puerto Real, Cádiz, Spain aut Gómez José Department of Chemical Engineering and Food Technologies, Faculty of Sciences, University of Cádiz, 11510, Puerto Real, Cádiz, Spain aut Cantero Domingo Department of Chemical Engineering and Food Technologies, Faculty of Sciences, University of Cádiz, 11510, Puerto Real, Cádiz, Spain aut Lafuente Javier Department of Chemical Engineering, School of Engineering, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut Gamisans Xavier Department of Mining Engineering and Natural Resources, Universitat Politècnica de Catalunya, Bases de Manresa 61-73, 08240, Manresa, Spain aut Gabriel David Department of Chemical Engineering, School of Engineering, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 77-87 0175-7598 99:1<77 2015 99 253 https://doi.org/10.1007/s00253-014-5688-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-5688-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mora Mabel Department of Chemical Engineering, School of Engineering, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut NATIONALLICENCE 700 1- Fernández Maikel Department of Chemical Engineering and Food Technologies, Faculty of Sciences, University of Cádiz, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- Gómez José Department of Chemical Engineering and Food Technologies, Faculty of Sciences, University of Cádiz, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- Cantero Domingo Department of Chemical Engineering and Food Technologies, Faculty of Sciences, University of Cádiz, 11510, Puerto Real, Cádiz, Spain aut NATIONALLICENCE 700 1- Lafuente Javier Department of Chemical Engineering, School of Engineering, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut NATIONALLICENCE 700 1- Gamisans Xavier Department of Mining Engineering and Natural Resources, Universitat Politècnica de Catalunya, Bases de Manresa 61-73, 08240, Manresa, Spain aut NATIONALLICENCE 700 1- Gabriel David Department of Chemical Engineering, School of Engineering, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 77-87 0175-7598 99:1<77 2015 99 253