caa a22 4500 60549794X CHVBK 20210128100543.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-5892-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-5892-3 Impact of mass transport on the enzymatic hydrolysis of rapeseed oil [Elektronische Daten] [Sandra Schröter, Klaus-Peter Stahmann, Klaus Schnitzlein] In order to assess the capillary segmented flow reactor as a potentially appropriate reactor device for the enzymatic hydrolysis of vegetable oils, a study was made to reveal the impact of incident mass transfer processes on the hydrolysis rate. As demonstrated by means of experiments performed in a modified Lewis-cell type contactor, which allows the independent adjustment of flow rates for both phases, the enzymatic hydrolysis rate of rapeseed oil is strongly governed by mass transport processes taking place in both phases. In the oil phase, any increase in convective mass transfer results in an enhancement of hydrolysis rate due to facilitated removal of fatty acids from interface layer which is known to inhibit the activity of the enzyme adsorbed at the interface. At asynchronous condition when solely the water phase is agitated, however, convective mass transport in the interface layer has an inverse effect on the hydrolysis rate due to the generation of considerable shear stress in the vicinity of the interface unfavorable for the performance of the enzymes. By operating at synchronous agitation conditions, the shear stress can considerably be reduced. Generally, the positive effect of mass transport in the oil phase compensates the negative one in the aqueous phase thus resulting in an overall increase in hydrolysis rate of 57 % with increasing stirrer rates. The results can be applied to the operation of segmented-flow capillary reactors by choosing the oil phase as disperse phase and the water phase as continuous phase, respectively. Springer-Verlag Berlin Heidelberg, 2014 Thermomyces lanuginosus lipase nationallicence Rapeseed oil nationallicence Mass transport nationallicence CFD simulation nationallicence Liquid-liquid segmented capillary flow nationallicence Schröter Sandra Chair of Chemical Reaction Engineering, Brandenburg University of Technology (BTU) Cottbus-Senftenberg, Burger Chaussee 2, 03044, Cottbus, Germany aut Stahmann Klaus-Peter Department of Biotechnology, BTU Cottbus-Senftenberg, Grossenhainer Str. 57, 01968, Senftenberg, Germany aut Schnitzlein Klaus Chair of Chemical Reaction Engineering, Brandenburg University of Technology (BTU) Cottbus-Senftenberg, Burger Chaussee 2, 03044, Cottbus, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 293-300 0175-7598 99:1<293 2015 99 253 https://doi.org/10.1007/s00253-014-5892-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-5892-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schröter Sandra Chair of Chemical Reaction Engineering, Brandenburg University of Technology (BTU) Cottbus-Senftenberg, Burger Chaussee 2, 03044, Cottbus, Germany aut NATIONALLICENCE 700 1- Stahmann Klaus-Peter Department of Biotechnology, BTU Cottbus-Senftenberg, Grossenhainer Str. 57, 01968, Senftenberg, Germany aut NATIONALLICENCE 700 1- Schnitzlein Klaus Chair of Chemical Reaction Engineering, Brandenburg University of Technology (BTU) Cottbus-Senftenberg, Burger Chaussee 2, 03044, Cottbus, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 293-300 0175-7598 99:1<293 2015 99 253