caa a22 4500 605497958 CHVBK 20210128100543.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-5868-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-5868-3 Investigating bacterial populations in styrene-degrading biofilters by 16S rDNA tag pyrosequencing [Elektronische Daten] [Kevin Portune, M. Pérez, F. Álvarez-Hornos, Carmen Gabaldón] Microbial biofilms are essential components in the elimination of pollutants within biofilters, yet still little is known regarding the complex relationships between microbial community structure and biodegradation function within these engineered ecosystems. To further explore this relationship, 16S rDNA tag pyrosequencing was applied to samples taken at four time points from a styrene-degrading biofilter undergoing variable operating conditions. Changes in microbial structure were observed between different stages of biofilter operation, and the level of styrene concentration was revealed to be a critical factor affecting these changes. Bacterial genera Azoarcus and Pseudomonas were among the dominant classified genera in the biofilter. Canonical correspondence analysis (CCA) and correlation analysis revealed that the genera Brevundimonas, Hydrogenophaga, and Achromobacter may play important roles in styrene degradation under increasing styrene concentrations. No significant correlations (P > 0.05) could be detected between biofilter operational/functional parameters and biodiversity measurements, although biological heterogeneity within biofilms and/or technical variability within pyrosequencing may have considerably affected these results. Percentages of selected bacterial taxonomic groups detected by fluorescence in situ hybridization (FISH) were compared to results from pyrosequencing in order to assess the effectiveness and limitations of each method for identifying each microbial taxon. Comparison of results revealed discrepancies between the two methods in the detected percentages of numerous taxonomic groups. Biases and technical limitations of both FISH and pyrosequencing, such as the binding of FISH probes to non-target microbial groups and lack of classification of sequences for defined taxonomic groups from pyrosequencing, may partially explain some differences between the two methods. The Author(s), 2014 Biofiltration nationallicence Styrene nationallicence Pyrosequencing nationallicence FISH nationallicence Portune Kevin Research Group GI2AM, Department of Chemical Engineering, Universitat de València, Av. de la Universidad s/n, 46100, Burjassot, Spain aut Pérez M. Research Group GI2AM, Department of Chemical Engineering, Universitat de València, Av. de la Universidad s/n, 46100, Burjassot, Spain aut Álvarez-Hornos F. Research Group GI2AM, Department of Chemical Engineering, Universitat de València, Av. de la Universidad s/n, 46100, Burjassot, Spain aut Gabaldón Carmen Research Group GI2AM, Department of Chemical Engineering, Universitat de València, Av. de la Universidad s/n, 46100, Burjassot, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 3-18 0175-7598 99:1<3 2015 99 253 https://doi.org/10.1007/s00253-014-5868-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-5868-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Portune Kevin Research Group GI2AM, Department of Chemical Engineering, Universitat de València, Av. de la Universidad s/n, 46100, Burjassot, Spain aut NATIONALLICENCE 700 1- Pérez M. Research Group GI2AM, Department of Chemical Engineering, Universitat de València, Av. de la Universidad s/n, 46100, Burjassot, Spain aut NATIONALLICENCE 700 1- Álvarez-Hornos F. Research Group GI2AM, Department of Chemical Engineering, Universitat de València, Av. de la Universidad s/n, 46100, Burjassot, Spain aut NATIONALLICENCE 700 1- Gabaldón Carmen Research Group GI2AM, Department of Chemical Engineering, Universitat de València, Av. de la Universidad s/n, 46100, Burjassot, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 3-18 0175-7598 99:1<3 2015 99 253