Influence of manufacturing processes on cell surface properties of probiotic strain Lactobacillus rhamnosus Lcr35®
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6110-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6110-z | ||
| 245 | 0 | 0 | |a Influence of manufacturing processes on cell surface properties of probiotic strain Lactobacillus rhamnosus Lcr35® |h [Elektronische Daten] |c [Adrien Nivoliez, Philippe Veisseire, Elina Alaterre, Caroline Dausset, Fabrice Baptiste, Olivier Camarès, Marylise Paquet-Gachinat, Muriel Bonnet, Christiane Forestier, Stéphanie Bornes] |
| 520 | 3 | |a The influence of the industrial process on the properties of probiotics, administered as complex manufactured products, has been poorly investigated. In the present study, we comparatively assessed the cell wall characteristics of the probiotic strain Lactobacillus rhamnosus Lcr35® together with three of its commercial formulations with intestinal applications. Putative secreted and transmembrane-protein-encoding genes were initially searched in silico in the genome of L. rhamnosus Lcr35®. A total of 369 candidate genes were identified which expressions were followed using a custom Lactobacillus DNA chip. Among them, 60 or 67 genes had their expression either upregulated or downregulated in the Lcr Restituo® packet or capsule formulations, compared to the native Lcr35® strain. Moreover, our data showed that the probiotic formulations (Lcr Lenio®, Lcr restituo® capsule and packet) showed a better capacity to adhere to intestinal epithelial Caco-2 cells than the native Lcr35® strain. Microbial (MATS) tests showed that the probiotic was an electron donor and that they were more hydrophilic than the native strain. The enhanced adhesion capacity of the active pharmaceutical ingredients (APIs) to epithelial Caco-2 cells and their antipathogen effect could be due to this greater surface hydrophilic character. These findings suggest that the manufacturing process influences the protein composition and the chemical properties of the cell wall. It is therefore likely that the antipathogen effect of the formulation is modulated by the industrial process. Screening of the manufactured products' properties would therefore represent an essential step in evaluating the effects of probiotic strains. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Probiotic |2 nationallicence | |
| 690 | 7 | |a Lactobacillus rhamnosus Lcr35® |2 nationallicence | |
| 690 | 7 | |a API manufacturing process |2 nationallicence | |
| 690 | 7 | |a Membrane proteins |2 nationallicence | |
| 690 | 7 | |a Surface properties |2 nationallicence | |
| 690 | 7 | |a Adhesion |2 nationallicence | |
| 700 | 1 | |a Nivoliez |D Adrien |u Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France |4 aut | |
| 700 | 1 | |a Veisseire |D Philippe |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | |
| 700 | 1 | |a Alaterre |D Elina |u Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France |4 aut | |
| 700 | 1 | |a Dausset |D Caroline |u Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France |4 aut | |
| 700 | 1 | |a Baptiste |D Fabrice |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | |
| 700 | 1 | |a Camarès |D Olivier |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | |
| 700 | 1 | |a Paquet-Gachinat |D Marylise |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | |
| 700 | 1 | |a Bonnet |D Muriel |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | |
| 700 | 1 | |a Forestier |D Christiane |u UMR CNRS 6023 Laboratoire Microorganismes: Génome Environnement (LMGE) Faculté de pharmacie, Clermont Université, 28, place H. Dunant, 63000, Clermont-Ferrand, France |4 aut | |
| 700 | 1 | |a Bornes |D Stéphanie |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 399-411 |x 0175-7598 |q 99:1<399 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6110-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6110-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nivoliez |D Adrien |u Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Veisseire |D Philippe |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Alaterre |D Elina |u Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dausset |D Caroline |u Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Baptiste |D Fabrice |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Camarès |D Olivier |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Paquet-Gachinat |D Marylise |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bonnet |D Muriel |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Forestier |D Christiane |u UMR CNRS 6023 Laboratoire Microorganismes: Génome Environnement (LMGE) Faculté de pharmacie, Clermont Université, 28, place H. Dunant, 63000, Clermont-Ferrand, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bornes |D Stéphanie |u Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 399-411 |x 0175-7598 |q 99:1<399 |1 2015 |2 99 |o 253 | ||