caa a22 4500 605497966 CHVBK 20210128100543.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6110-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-6110-z Influence of manufacturing processes on cell surface properties of probiotic strain Lactobacillus rhamnosus Lcr35® [Elektronische Daten] [Adrien Nivoliez, Philippe Veisseire, Elina Alaterre, Caroline Dausset, Fabrice Baptiste, Olivier Camarès, Marylise Paquet-Gachinat, Muriel Bonnet, Christiane Forestier, Stéphanie Bornes] The influence of the industrial process on the properties of probiotics, administered as complex manufactured products, has been poorly investigated. In the present study, we comparatively assessed the cell wall characteristics of the probiotic strain Lactobacillus rhamnosus Lcr35® together with three of its commercial formulations with intestinal applications. Putative secreted and transmembrane-protein-encoding genes were initially searched in silico in the genome of L. rhamnosus Lcr35®. A total of 369 candidate genes were identified which expressions were followed using a custom Lactobacillus DNA chip. Among them, 60 or 67 genes had their expression either upregulated or downregulated in the Lcr Restituo® packet or capsule formulations, compared to the native Lcr35® strain. Moreover, our data showed that the probiotic formulations (Lcr Lenio®, Lcr restituo® capsule and packet) showed a better capacity to adhere to intestinal epithelial Caco-2 cells than the native Lcr35® strain. Microbial (MATS) tests showed that the probiotic was an electron donor and that they were more hydrophilic than the native strain. The enhanced adhesion capacity of the active pharmaceutical ingredients (APIs) to epithelial Caco-2 cells and their antipathogen effect could be due to this greater surface hydrophilic character. These findings suggest that the manufacturing process influences the protein composition and the chemical properties of the cell wall. It is therefore likely that the antipathogen effect of the formulation is modulated by the industrial process. Screening of the manufactured products' properties would therefore represent an essential step in evaluating the effects of probiotic strains. Springer-Verlag Berlin Heidelberg, 2014 Probiotic nationallicence Lactobacillus rhamnosus Lcr35® nationallicence API manufacturing process nationallicence Membrane proteins nationallicence Surface properties nationallicence Adhesion nationallicence Nivoliez Adrien Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France aut Veisseire Philippe Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut Alaterre Elina Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France aut Dausset Caroline Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France aut Baptiste Fabrice Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut Camarès Olivier Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut Paquet-Gachinat Marylise Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut Bonnet Muriel Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut Forestier Christiane UMR CNRS 6023 Laboratoire Microorganismes: Génome Environnement (LMGE) Faculté de pharmacie, Clermont Université, 28, place H. Dunant, 63000, Clermont-Ferrand, France aut Bornes Stéphanie Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 399-411 0175-7598 99:1<399 2015 99 253 https://doi.org/10.1007/s00253-014-6110-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6110-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nivoliez Adrien Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France aut NATIONALLICENCE 700 1- Veisseire Philippe Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Alaterre Elina Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France aut NATIONALLICENCE 700 1- Dausset Caroline Département Recherche et Développement-Probionov, Rue des frères Lumières, 15130, Arpajon-sur-Cère, France aut NATIONALLICENCE 700 1- Baptiste Fabrice Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Camarès Olivier Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Paquet-Gachinat Marylise Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Bonnet Muriel Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Forestier Christiane UMR CNRS 6023 Laboratoire Microorganismes: Génome Environnement (LMGE) Faculté de pharmacie, Clermont Université, 28, place H. Dunant, 63000, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Bornes Stéphanie Laboratoire de Biologie, Clermont Université, Université d'Auvergne, IUT Aurillac, BP 10448, 63000, Clermont-Ferrand, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 399-411 0175-7598 99:1<399 2015 99 253