ClaR—a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605497990 | ||
| 003 | CHVBK | ||
| 005 | 20210128100544.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6067-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6067-y | ||
| 245 | 0 | 0 | |a ClaR—a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403 |h [Elektronische Daten] |c [Tamara Aleksandrzak-Piekarczyk, Lidia Stasiak-Różańska, Jarosław Cieśla, Jacek Bardowski] |
| 520 | 3 | |a In a number of previous studies, our group has discovered an alternative pathway for lactose utilization in Lactococcus lactis that, in addition to a sugar-hydrolyzing enzyme with both P-β-glucosidase and P-β-galactosidase activity (BglS), engages chromosomally encoded components of cellobiose-specific PTS (PTSCel-Lac), including PtcA, PtcB, and CelB. In this report, we show that this system undergoes regulation via ClaR, a novel activator protein from the RpiR family of transcriptional regulators. Although RpiR proteins are widely distributed among lactic acid bacteria, their roles have yet to be confirmed by functional assays. Here, we show that ClaR activity depends on intracellular cellobiose-6-phosphate availability, while other sugars such as glucose or galactose have no influence on it. We also show that ClaR is crucial for activation of the bglS and celB expression in the presence of cellobiose, with some limited effects on ptcA and ptcB activation. Among 190 of carbon sources tested, the deletion of claR reduces L. lactis growth only in lactose- and/or cellobiose-containing media, suggesting a narrow specificity of this regulator within the context of sugar metabolism. | |
| 540 | |a The Author(s), 2014 | ||
| 690 | 7 | |a Lactoseassimilation |2 nationallicence | |
| 690 | 7 | |a Cellobiose assimilation |2 nationallicence | |
| 690 | 7 | |a Phosphotransferase system (PTS) |2 nationallicence | |
| 690 | 7 | |a Genes regulation |2 nationallicence | |
| 690 | 7 | |a Lactococcus lactis |2 nationallicence | |
| 700 | 1 | |a Aleksandrzak-Piekarczyk |D Tamara |u Institute of Biochemistry and Biophysics, Polish Academy of Sciences (IBB PAS), Pawińskiego 5a, 02-106, Warsaw, Poland |4 aut | |
| 700 | 1 | |a Stasiak-Różańska |D Lidia |u Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776, Warsaw, Poland |4 aut | |
| 700 | 1 | |a Cieśla |D Jarosław |u Institute of Biochemistry and Biophysics, Polish Academy of Sciences (IBB PAS), Pawińskiego 5a, 02-106, Warsaw, Poland |4 aut | |
| 700 | 1 | |a Bardowski |D Jacek |u Institute of Biochemistry and Biophysics, Polish Academy of Sciences (IBB PAS), Pawińskiego 5a, 02-106, Warsaw, Poland |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 337-347 |x 0175-7598 |q 99:1<337 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6067-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6067-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Aleksandrzak-Piekarczyk |D Tamara |u Institute of Biochemistry and Biophysics, Polish Academy of Sciences (IBB PAS), Pawińskiego 5a, 02-106, Warsaw, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Stasiak-Różańska |D Lidia |u Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776, Warsaw, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cieśla |D Jarosław |u Institute of Biochemistry and Biophysics, Polish Academy of Sciences (IBB PAS), Pawińskiego 5a, 02-106, Warsaw, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bardowski |D Jacek |u Institute of Biochemistry and Biophysics, Polish Academy of Sciences (IBB PAS), Pawińskiego 5a, 02-106, Warsaw, Poland |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 337-347 |x 0175-7598 |q 99:1<337 |1 2015 |2 99 |o 253 | ||