Metabolic engineering of itaconate production in Escherichia coli
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6092-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6092-x | ||
| 245 | 0 | 0 | |a Metabolic engineering of itaconate production in Escherichia coli |h [Elektronische Daten] |c [Kiira Vuoristo, Astrid Mars, Jose Sangra, Jan Springer, Gerrit Eggink, Johan Sanders, Ruud Weusthuis] |
| 520 | 3 | |a Interest in sustainable development has led to efforts to replace petrochemical-based monomers with biomass-based ones. Itaconic acid, a C5-dicarboxylic acid, is a potential monomer for the chemical industry with many prospective applications. cis-aconitate decarboxylase (CadA) is the key enzyme of itaconate production, converting the citric acid cycle intermediate cis-aconitate into itaconate. Heterologous expression of cadA from Aspergillus terreus in Escherichia coli resulted in low CadA activities and production of trace amounts of itaconate on Luria-Bertani (LB) medium (<10mg/L). CadA was primarily present as inclusion bodies, explaining the low activity. The activity was significantly improved by using lower cultivation temperatures and mineral medium, and this resulted in enhanced itaconate titres (240mg/L). The itaconate titre was further increased by introducing citrate synthase and aconitase from Corynebacterium glutamicum and by deleting the genes encoding phosphate acetyltransferase and lactate dehydrogenase. These deletions in E. coli's central metabolism resulted in the accumulation of pyruvate, which is a precursor for itaconate biosynthesis. As a result, itaconate production in aerobic bioreactor cultures was increased up to 690mg/L. The maximum yield obtained was 0.09mol itaconate/mol glucose. Strategies for a further improvement of itaconate production are discussed. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Itaconic acid |2 nationallicence | |
| 690 | 7 | |a Escherichia coli |2 nationallicence | |
| 690 | 7 | |a Metabolic engineering |2 nationallicence | |
| 690 | 7 | |a Citrate synthase |2 nationallicence | |
| 690 | 7 | |a Aconitase |2 nationallicence | |
| 690 | 7 | |a cis -aconitate decarboxylase |2 nationallicence | |
| 700 | 1 | |a Vuoristo |D Kiira |u Bioprocess Engineering, Wageningen University, Wageningen, The Netherlands |4 aut | |
| 700 | 1 | |a Mars |D Astrid |u Biobased Products, Wageningen University and Research Centre, Wageningen UR PO Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | |
| 700 | 1 | |a Sangra |D Jose |u Bioprocess Engineering, Wageningen University, Wageningen, The Netherlands |4 aut | |
| 700 | 1 | |a Springer |D Jan |u Biobased Products, Wageningen University and Research Centre, Wageningen UR PO Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | |
| 700 | 1 | |a Eggink |D Gerrit |u Biobased Products, Wageningen University and Research Centre, Wageningen UR PO Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | |
| 700 | 1 | |a Sanders |D Johan |u Biobased Products, Wageningen University and Research Centre, Wageningen UR PO Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | |
| 700 | 1 | |a Weusthuis |D Ruud |u Bioprocess Engineering, Wageningen University, Wageningen, The Netherlands |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 221-228 |x 0175-7598 |q 99:1<221 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6092-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6092-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vuoristo |D Kiira |u Bioprocess Engineering, Wageningen University, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mars |D Astrid |u Biobased Products, Wageningen University and Research Centre, Wageningen UR PO Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sangra |D Jose |u Bioprocess Engineering, Wageningen University, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Springer |D Jan |u Biobased Products, Wageningen University and Research Centre, Wageningen UR PO Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Eggink |D Gerrit |u Biobased Products, Wageningen University and Research Centre, Wageningen UR PO Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sanders |D Johan |u Biobased Products, Wageningen University and Research Centre, Wageningen UR PO Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Weusthuis |D Ruud |u Bioprocess Engineering, Wageningen University, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/1(2015-01-01), 221-228 |x 0175-7598 |q 99:1<221 |1 2015 |2 99 |o 253 | ||