caa a22 4500 605498024 CHVBK 20210128100544.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-6100-1 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6100-1 Efficient pullulan production by bioconversion using Aureobasidium pullulans as the whole-cell catalyst [Elektronische Daten] [Xiao-Min Ju, Da-Hui Wang, Gao-Chuan Zhang, Dan Cao, Gong-Yuan Wei] In this study, pullulan production was achieved by whole-cell bioconversion with Aureobasidium pullulans CCTCC M 2012259. Response surface methodology was applied to optimize the seed medium for incubating cells with high capability of pullulan bioconversion. Three medium components, namely, yeast extract, MgSO4·7H2O, and glucose were identified by Plackett-Berman design as significant factors affecting the cells' pullulan bioconversion capability. A three-level Box-Behnken design was then employed to determine the optimal levels of the three components. A mathematical model was developed to show the influence of each medium component and its effects on the cells' pullulan bioconversion capability. The model predicted a maximum pullulan bioconversion capability of 32.28mg/g/h at the optimal yeast extract, MgSO4·7H2O, and glucose concentrations of 3.57, 0.18, and 63.97g/l, respectively. The validation experiments showed that the cells' pullulan bioconversion capability was improved by 23.1% when the optimal medium was used, as compared with that obtained with the basic medium. Subsequently, the gene expression and activities of the key enzymes involved in pullulan biosynthesis were evaluated. When the optimal medium was employed, the transcriptional levels of pgm1 and fks were up-regulated by 2.5- and 1.2-fold, respectively, and the α-phosphoglucose mutase and glucosyltransferase activities were increased by 17 and 19%, respectively, when compared with those achieved using the basic medium. These results indicated that pullulan bioconversion using A. pullulans CCTCC M 2012259 as the whole-cell catalyst is an attractive approach for efficient pullulan production and can be applied for the production of other polysaccharides. Springer-Verlag Berlin Heidelberg, 2014 Pullulan nationallicence Bioconversion nationallicence Aureobasidium pullulans nationallicence Response surface methodology nationallicence Whole-cell catalyst nationallicence Ju Xiao-Min School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut Wang Da-Hui School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut Zhang Gao-Chuan School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut Cao Dan School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut Wei Gong-Yuan School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 211-220 0175-7598 99:1<211 2015 99 253 https://doi.org/10.1007/s00253-014-6100-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6100-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ju Xiao-Min School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Da-Hui School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Gao-Chuan School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cao Dan School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wei Gong-Yuan School of Biology and Basic Medical Sciences, Soochow University, 199 Ren-Ai Road, 215123, Suzhou, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 211-220 0175-7598 99:1<211 2015 99 253