caa a22 4500 605498067 CHVBK 20210128100544.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00253-014-5960-8 doi (NATIONALLICENCE)springer-10.1007/s00253-014-5960-8 Diversity analysis of the bacterial community in tobacco waste extract during reconstituted tobacco process [Elektronische Daten] [Huagui Liu, Houlong He, Changhe Cheng, Jinli Liu, Ming Shu, Yang Jiao, Feng Tao, Weihong Zhong] Reconstituted tobacco sheet process has been developed to treat and reuse tobacco wastes in the industry. During this process, microorganisms in original and concentrated tobacco waste extract (TWE) might play important roles in the final quality of the reconstituted tobacco. However, microbial communities in TWE remain largely unknown. In the present study, the Roche 454bar-coded pyrosequencing was applied to analyze the bacterial community structure in samples. Comparison based on 16S rRNA gene sequences showed that the original and concentrated solutions of TWE harbored abundant bacteria probably resistant to the acid, high nicotine concentration, and high osmotic pressure environment. The dominant phyla were Firmicutes and Proteobacteria. Lactobacillus and Lysinibacillus were the dominant genera of Firmicutes. The most interesting genus of Proteobacteria was Pseudomonas. It is the first time to reveal the bacterial diversities on the TWE samples from the process of reconstituted tobacco sheets. Springer-Verlag Berlin Heidelberg, 2014 Reconstituted tobacco nationallicence Tobacco waste extract nationallicence 16S rRNA gene nationallicence Bacterial diversity nationallicence Liu Huagui College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut He Houlong College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut Cheng Changhe Technology Center, China Tobacco Zhejiang Industrial Co., Ltd., 310009, Hangzhou, People's Republic of China aut Liu Jinli Technology Center, China Tobacco Zhejiang Industrial Co., Ltd., 310009, Hangzhou, People's Republic of China aut Shu Ming Technology Center, China Tobacco Zhejiang Industrial Co., Ltd., 310009, Hangzhou, People's Republic of China aut Jiao Yang College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut Tao Feng Technology Center, Hangzhou Liqun Environment Protecting Paper Co., Ltd, 310018, Hangzhou, People's Republic of China aut Zhong Weihong College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 469-476 0175-7598 99:1<469 2015 99 253 https://doi.org/10.1007/s00253-014-5960-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-5960-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Huagui College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- He Houlong College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cheng Changhe Technology Center, China Tobacco Zhejiang Industrial Co., Ltd., 310009, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liu Jinli Technology Center, China Tobacco Zhejiang Industrial Co., Ltd., 310009, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Shu Ming Technology Center, China Tobacco Zhejiang Industrial Co., Ltd., 310009, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Jiao Yang College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Tao Feng Technology Center, Hangzhou Liqun Environment Protecting Paper Co., Ltd, 310018, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhong Weihong College of Biological and Environmental Engineering, Zhejiang University of Technology, 310032, Hangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/1(2015-01-01), 469-476 0175-7598 99:1<469 2015 99 253